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PMID: 6265904 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Specific binding of a prokaryotic ribosomal protein to a eukaryotic ribosomal RNA: implications for evolution and autoregulation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 2722-6

Gourse RL, Thurlow DL, Gerbi SA, Zimmermann RA

Abstract

Ribosomal protein L1 from the prokaryote Escherichia coli has been shown to form a specific complex with 26S ribosomal RNA from the eukaryote Dictyostelium discoideum. The segment of Dictyostelium rRNA protected from ribonuclease digestion by L1 and the corresponding region in Dictyostelium rDNA were investigated by nucleotide sequence analysis, and an analogous section in rDNA from Xenopus laevis was identified. When the L1-specific segments from eukaryotic rRNA were compared with those from prokaryotic rRNA, striking similarities in both primary and secondary structure were apparent. These conserved features suggest a common structural basis for protein recognition and indicate that such regions became fixed at a very early stage in rRNA evolution. In addition, certain structural elements of the L1 binding sites in rRNA are also found in the initial segment of the polycistronic L11-L1 mRNA, providing support for the hypothesis that L1 participates in the regulation of ribosomal protein synthesis by specific interaction with its own mRNA.

MeSH Terms
Base Sequence Binding Sites Biological Evolution DNA Restriction Enzymes Dictyostelium/metabolism Escherichia coli/metabolism Kinetics Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Ribosomal/metabolism Species Specificity
Chemicals
RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gourse R L
Thurlow D L
Gerbi S A
Zimmermann R A
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.2722
Published
1981-05-00
Pages
2722-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319429
Subset
IM
Grants
NIGMS NIH HHS · GM 00036 · United States
NIGMS NIH HHS · GM 07601 · United States
NIGMS NIH HHS · GM20261 · United States
Databases
GENBANK
M10365, M10369
External Links
PubMed source DOI: 10.1073/pnas.78.5.2722 PMC Full Text View journal details
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