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PMID: 6265907 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 5 ·1981-05-00 ·Pages 2747-51

Sternglanz R, DiNardo S, Voelkel KA, Nishimura Y, Hirota Y, Becherer K, Zumstein L, Wang JC

Abstract

Mutations in top, the structural gene for Escherichia coli DNA topoisomerase I, have been identified and mapped at 28 min on the chromosome, near cysB. Strains carrying deletions of the top gene are viable. The top mutations, however, do exert pleiotropic effects on transcription and transposition. Mutants lacking DNA topoisomerase I have a more rapid rate of induction and a higher level of catabolite-sensitive enzymes including tryptophanase and beta-galactosidase. This general activation of transcription by top mutations can be attributed to an increase in the negative superhelicity of the DNA in vivo when the topoisomerase activity is abolished. The frequency of transposition of Tn5, a transposon carrying kanamycin resistance, is decreased by a factor of 40 or more in top mutants. A direct or indirect role of the topoisomerase in transposition is discussed. The transposition frequency of Tn3, however, is not dependent on top. Based on the studies of the E. coli top mutants, it appears that the supX gene, which was originally studied in Salmonella typhimurium [Dubnau, E. & Margolin, P. (1972) Mol. Gen. Genet. 117, 91-112] is likely to be the structural gene for DNA topoisomerase I.

MeSH Terms
Chromosomes, Bacterial/metabolism DNA Topoisomerases, Type I/genetics Enzyme Induction Escherichia coli/enzymology,genetics Genes Genotype Mutation Recombination, Genetic Transcription, Genetic Tryptophanase/genetics beta-Galactosidase/genetics
Chemicals
beta-Galactosidase Tryptophanase DNA Topoisomerases, Type I
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sternglanz R
DiNardo S
Voelkel K A
Nishimura Y
Hirota Y
Becherer K
Zumstein L
Wang J C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.5.2747
Published
1981-05-00
Pages
2747-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319434
Subset
IM
Grants
NIGMS NIH HHS · GM 24544 · United States
NIGMS NIH HHS · GM 28220 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.5.2747 PMC Full Text View journal details
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