pED100, a conjugative F plasmid derivative without insertion sequences.

Molecular & general genetics : MGG ·Vol. 182 ·No. 3 ·1981-00-00 ·Pages 520-2

Willetts N, Johnson D

Abstract

The largest HindIII fragment of F includes the entire replication and transfer regions, and its circularisation with ligase gave the conjugative plasmid pED100. This plasmid, which contains none of the F insertion sequences, was essentially unable to mobilise the E. coli chromosome or to give integrative suppression of a dnaA strain.

MeSH Terms

Chromosome Mapping Conjugation, Genetic DNA Restriction Enzymes/metabolism DNA Transposable Elements DNA, Bacterial/genetics Escherichia coli/genetics F Factor Recombination, Genetic

Chemicals

DNA Transposable Elements DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Willetts N

Johnson D

References (20)

20 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00293948

Published

1981-00-00

Pages

520-2

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00293948

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pED100, a conjugative F plasmid derivative without insertion sequences.

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