Home LiteratureArticle Details
PMID: 6272283 Published · ppublish English Journal Article

Identification and cloning of endogenous retroviral sequences present in human DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 8 ·1981-08-00 ·Pages 4892-6

Martin MA, Bryan T, Rasheed S, Khan AS

Abstract

Using nonstringent annealing conditions and a 2.75-kilobase segment of cloned African green monkey DNA that specifically hybridizes to the proviruses of AKR ecotropic murine leukemia virus (MuLV) and baboon endogenous virus (BaEV) as a probe, we detected related sequences in three different preparations of human brain DNA fragments. The blot-hybridization pattern obtained with cleaved human DNA was similar to that previously reported for the interaction of MuLV cDNA and cleaved mouse DNA and suggested the presence of numerous copies of retrovirus-related sequences in the human genome. The labeled 2.75-kilobase fragment derived from cloned monkey DNA was used to screen a human DNA library in Charon 4A. One clone obtained hybridized to three contiguous MuLV-and BaEV-reactive fragments of the cloned monkey DNA and to multiple fragments of human DNA including a prominent 1.0-kilobase EcoRI fragment also present in the clone.

MeSH Terms
Animals Bacteriophage lambda/genetics Base Sequence Brain Cell Transformation, Viral Chlorocebus aethiops/microbiology Cloning, Molecular/methods DNA, Viral/genetics Humans Leukemia Virus, Murine/genetics Retroviridae/genetics
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Martin M A
Bryan T
Rasheed S
Khan A S
References (36)
36 references, click to expand
  1. Genetic mapping of xenotropic leukemia virus-inducing loci in two mouse strains.
    Science. 1978 Mar 31;199(4336):1448-9 PMID: 204014
  2. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  3. Radioimmunologic characterization of RD-114 reverse transcriptase: evolutionary relatedness of mammalian type C viral pol gene products.
    Virology. 1978 May 1;86(1):127-37 PMID: 78564
  4. Leukemia virus genomes in the chromosomal DNA of the mouse.
    Harvey Lect. 1978;71:173-92 PMID: 213400
  5. Evaluation of normal and neoplastic human tissue for BK virus.
    Virology. 1978 Oct 15;90(2):187-96 PMID: 214941
  6. The integrated genome of murine leukemia virus.
    Cell. 1978 Nov;15(3):1003-10 PMID: 728982
  7. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  8. Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system.
    Science. 1979 Mar 2;203(4383):883-7 PMID: 217087
  9. Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning.
    J Virol. 1979 Feb;29(2):555-75 PMID: 372561
  10. Genetic mapping of the ecotropic murine leukemia virus-inducing locus of BALB/c mouse to chromosome 5.
    Science. 1979 Apr 6;204(4388):69-71 PMID: 219475
  11. Genetic linkage of C3H/HeJ and BALB/c endogenous ecotropic C-type viruses to phosphoglucomutase-1 on chromosome 5.
    Science. 1979 Apr 6;204(4388):71-3 PMID: 219476
  12. Isolation and characterization of an endogenous type C virus of rhesus monkeys.
    Science. 1979 May 25;204(4395):841-2 PMID: 87013
  13. Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts.
    J Virol. 1979 Jun;30(3):657-67 PMID: 480464
  14. A new endogenous primate type C virus isolated from the Old World monkey Colobus polykomos.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5041-5 PMID: 228275
  15. Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):614-8 PMID: 6244569
  16. Characterization of polyoma viral DNA sequences in polyoma-induced hamster tumor cell lines.
    J Biol Chem. 1980 Apr 25;255(8):3798-805 PMID: 6245092
  17. The evolution of baboon endogenous type C virus: related sequences in the DNA of distant species.
    Virology. 1980 May;103(1):217-27 PMID: 6245528
  18. Baboon endogenous virus genome. I. Restriction enzyme map of the unintegrated DNA genome of a primate retrovirus.
    J Virol. 1980 Apr;34(1):28-39 PMID: 6246259
  19. The baboon endogenous virus genome. II. Provirus sequence variations in baboon cell DNA.
    Nucleic Acids Res. 1980 Oct 10;8(19):4423-40 PMID: 6253918
  20. Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5779-83 PMID: 6255465
  21. Analysis of the virogenes related to the rhesus monkey endogenous type C retrovirus in monkeys and apes.
    J Virol. 1981 Mar;37(3):922-30 PMID: 6262537
  22. Pathogenic properties, and "vertical" transmission of the mouse leukemia agent.
    Proc Soc Exp Biol Med. 1951 Oct;78(1):342-8 PMID: 14892014
  23. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome.
    J Exp Med. 1971 Jun 1;133(6):1334-55 PMID: 5576334
  24. Genetic mapping of a murine leukemia virus-inducing locus of AKR mice.
    Science. 1972 Nov 24;178(4063):860-2 PMID: 4343696
  25. Genetic factors in the natural history of murine leukemia virus infection: G. H. A. Clowes Memorial Lecture.
    Cancer Res. 1973 Dec;33(12):3061-8 PMID: 4357347
  26. Characterization of a newly derived human sarcoma cell line (HT-1080).
    Cancer. 1974 Apr;33(4):1027-33 PMID: 4132053
  27. Detection of baboon type C viral sequences in various primate tissues by molecular hybridization.
    J Virol. 1974 Jul;14(1):56-67 PMID: 4365323
  28. Type C viruses of baboons: isolation from normal cell cultures.
    Cell. 1974 May;2(1):55-61 PMID: 4138028
  29. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  30. Qualitative and quantitative studies of AKR-type murine leukemia virus sequences in mouse DNA.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1085-101 PMID: 169010
  31. Endogenous primate and feline type C viruses.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1159-68 PMID: 50895
  32. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  33. Evolution of type C viral genes: evidence for an Asian origin of man.
    Nature. 1976 May 13;261(5556):101-8 PMID: 179013
  34. Proviral sequences of baboon endogenous type C RNA virus in DNA of human leukaemic tissues.
    Nature. 1976 JUL 15;262(5565):190-5 PMID: 180434
  35. Evolutionary relationships between gag gene-coded proteins of murine and primate endogenous type C RNA viruses.
    Cell. 1977 Apr;10(4):641-8 PMID: 67898
  36. MAC-1, a new genetically transmitted type C virus of primates: "low frequency" activation from stumptail monkey cell cultures.
    Cell. 1978 Apr;13(4):775-82 PMID: 207440
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.8.4892
Published
1981-08-00
Pages
4892-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC320286
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.8.4892 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2