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PMID: 6272302 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Gene II of phage f1: its functions and its products.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 9 ·1981-09-00 ·Pages 5421-24

Dotto GP, Enea V, Zinder ND

Abstract

Plasmids harboring the amino-terminal part of bacteriophage f1 gene II confer to bacterial cells partial resistance to infection with the male-specific bacteriophages f1 and f2. This effect (IP-2 phenotype) is due to the production of large amounts of an approximately 20,000-dalton polypeptide corresponding to the amino-terminal part of gene II protein. These results have allowed the isolation of clones producing functional gene II protein in large amounts. An in vitro assay has been developed to test the enzymatic activity of the gene II protein produced.

MeSH Terms

Cloning, Molecular/methods Coliphages/genetics DNA, Superhelical/metabolism DNA, Viral/biosynthesis Endonucleases/genetics Genes Genes, Viral Plasmids Viral Proteins/genetics Virus Replication

Chemicals

DNA, Superhelical DNA, Viral Viral Proteins Endonucleases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Dotto G P

Enea V

Zinder N D

References (28)

28 references, click to expand

Filamentous bacterial viruses.
Bacteriol Rev. 1969 Jun;33(2):172-209 PMID: 4979697
Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
Gene. 1978 Oct;4(2):121-36 PMID: 363519
Replication of bacteriophage M13. VI. Attachment of M13 DNA to a fast-sedimenting host cell component.
Virology. 1971 Mar;43(3):647-64 PMID: 4940969
The genetic map of the filamentous bacteriophage f1.
Virology. 1972 Jul;49(1):45-60 PMID: 4556929
Abortive infection of Escherichia coli with the bacteriophage f1: DNA synthesis associated with the membrane.
Virology. 1973 Sep;55(1):39-52 PMID: 4580738
The identification of prereplicative bacteriophage T4 proteins.
J Biol Chem. 1973 Aug 10;248(15):5499-501 PMID: 4588684
Resistance to colicins E3 and K induced by infection with bacteriophage f1.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3160-4 PMID: 4594036
In vitro synthesis of bacteriophage f1 proteins.
J Mol Biol. 1974 Feb 25;83(2):231-51 PMID: 4595423
Bacteriophage M 13 gene 2 protein: increasing its yield in infected cells, and identification and localization.
Virology. 1974 Oct;61(2):334-42 PMID: 4608080
Endonuclease R-EcoRII restriction of bacteriophage f1 DNA in vitro: ordering of genes V and VII, location of an RNA promotor for gene VIII.
J Virol. 1975 Sep;16(3):674-84 PMID: 1159896
Cleavage site of bacteriophage fd gene II-protein in the origin of viral strand replication.
Nature. 1979 Mar 22;278(5702):365-7 PMID: 423991
Insertion mutant of bacteriophage f1 sensitive to EcoRI.
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2699-702 PMID: 379863
Insertion mutations affecting transposition of Tn3 and replication of a ColE1 derivative.
Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1279-85 PMID: 385228
Bacteriophage fd gene II-protein. I. Purification, involvement in RF replication, and the expression of gene II.
J Biol Chem. 1979 Dec 25;254(24):12636-41 PMID: 387791
Bacteriophage fd gene II-protein. II. Specific cleavage and relaxation of supercoiled RF from filamentous phages.
J Biol Chem. 1979 Dec 25;254(24):12642-6 PMID: 387792
Specific recombination in vitro promoted by the restriction endonuclease HgaI.
J Mol Biol. 1979 Dec 5;135(2):517-24 PMID: 231678
Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.
Cell. 1980 Jun;20(2):543-53 PMID: 6248249
Replication of the plasmid pBR322 under the control of a cloned replication origin from the single-stranded DNA phage M13.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4638-42 PMID: 6933512
Replication of bacteriophage f1: a complex containing gene II protein in gene V mutant-infected bacteria.
J Mol Biol. 1980 May 25;139(3):393-405 PMID: 7441740
Processing of filamentous phage pre-coat protein. Effect of sequence variations near the signal peptidase cleavage site.
J Mol Biol. 1980 Dec 5;144(2):103-16 PMID: 7230262
One and two codon insertion mutants of bacteriophage f1.
Mol Gen Genet. 1981;181(3):288-91 PMID: 6264271
Cloning of bacteriophage fd gene 2 and construction of a plasmid dependent on fd gene 2 protein.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5416-20 PMID: 6272301
Cleavage map of bacteriophage f1: location of the Escherichia coli B-specific modification sites.
J Mol Biol. 1975 Jun 25;95(2):147-65 PMID: 1102704
A delection mutant of bacteriophage f1 containing no intact cistrons.
Virology. 1975 Nov;68(1):105-14 PMID: 52943
The single-stranded DNA phages.
CRC Crit Rev Microbiol. 1975 Dec;4(2):161-223 PMID: 1106952
Nucleotide sequences near the origin of replication of bacteriophage f1.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4219-22 PMID: 270666
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Gene. 1977;2(2):95-113 PMID: 344137
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.9.5421

Published

1981-09-00

Pages

5421-24

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348757

Subset

External Links

PubMed source DOI: 10.1073/pnas.78.9.5421 PMC Full Text View journal details

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