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PMID: 6272736 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phorbol esters induce synthesis of thromboplastin activity in human monocytes.

The Biochemical journal ·Vol. 194 ·No. 3 ·1981-03-15 ·Pages 699-706

Lyberg T, Prydz H

Abstract

12-O-Tetradecanoylphorbol 13-acetate (TPA), phorbol 12,13-diacetate and phorbol 12,13-didecanoate were all potent inducers of thromboplastin activity in human monocytes in vitro, whereas 4 alpha-phorbol 12,13-didecanoate and 4 alpha-phorbol had no such effect. A concomitant increase in titrable apoprotein III antigen was found (apoprotein III is the protein component of thromboplastin). The increase was inhibited by cycloheximide and actinomycin D and partly by alpha-amanitin. The increase of thromboplastin activity was therefore most likely due to synthesis de novo of apoprotein III. The response was approximately halved in the absence of serum or Ca2+. Retinol had a weak inhibitory effect, and retinoic acid was inhibitory only at concentrations that also induced signs of cytotoxicity. TPA caused an initial rise in monocyte cyclic AMP concentration of about 90-120 min duration. No increase in 45Ca2+ influx was induced over 2 h. Good correlation exists between induction of apoprotein III synthesis in monocytes in vitro and mouse skin-tumour promotion in vivo by the various phorbol derivatives. Substances inactive in tumour promotion do not induce the synthesis of apoprotein III. General activating and cytotoxic effects of TPA were monitored by determining release of lysozyme, beta-glucuronidase and lactate dehydrogenase.

MeSH Terms
Apoproteins/biosynthesis Cells, Cultured Cyclic AMP/metabolism Dose-Response Relationship, Drug Humans Monocytes/drug effects,enzymology,metabolism Phorbol Esters/pharmacology Phorbols/pharmacology Tetradecanoylphorbol Acetate/pharmacology Thromboplastin/biosynthesis
Chemicals
Apoproteins Phorbol Esters Phorbols Thromboplastin Cyclic AMP Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lyberg T
Prydz H
References (49)
49 references, click to expand
  1. In vitro synthesis and secretion of lysozyme by mononuclear phagocytes.
    J Exp Med. 1974 May 1;139(5):1228-48 PMID: 4825244
  2. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  3. The influence of phorbol myristate acetate on oxygen consumption by polymorphonuclear leukocytes.
    J Lab Clin Med. 1974 Jun;83(6):911-20 PMID: 4421315
  4. Synergistic action of phorbol esters in mitogen-activated bovine lymphocytes.
    Exp Cell Res. 1974 Sep;88(1):40-6 PMID: 4609010
  5. The endotoxin-induced coagulant activity of human monocytes.
    Br J Haematol. 1975 Jul;30(3):311-6 PMID: 1201214
  6. Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells.
    Cancer Res. 1976 Jul;36(7 PT 1):2254-60 PMID: 819128
  7. Macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides.
    Cell. 1976 Jun;8(2):271-81 PMID: 61067
  8. Phorbol myristate acetate: a mitogen selective for a T-lymphocyte subpopulation.
    J Exp Med. 1977 Feb 1;145(2):460-5 PMID: 299884
  9. Leukocyte procoagulant activity: enhancement of production in vitro by IgG and antigen-antibody complexes.
    J Clin Invest. 1977 Mar;59(3):549-57 PMID: 190271
  10. The effect of phorbol diesters on chicken embryo fibroblasts.
    Cancer Res. 1977 Sep;37(9):3257-65 PMID: 195722
  11. Purification and some properties of the protein component of tissue thromboplastin from human brain.
    Biochem J. 1977 Jul 1;165(1):89-96 PMID: 889578
  12. Tumor promoters inhibit spontaneous differentiation of Friend erythroleukemia cells in culture.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2894-8 PMID: 268640
  13. Tumor promoters inhibit spontaneous and induced differentiation of murine erythroleukemia cells in culture.
    Proc Natl Acad Sci U S A. 1977 Aug;74(8):3451-5 PMID: 269404
  14. Secretion of plasminogen activator by human polymorphonuclear leukocytes. Modulation by glucocorticoids and other effectors.
    J Exp Med. 1977 Dec 1;146(6):1693-706 PMID: 200699
  15. Retinoic acid inhibition of the comitogenic action of mezerein and phorbol esters in bovine lymphocytes.
    Cancer Res. 1978 Mar;38(3):771-5 PMID: 626980
  16. Induction of plasminogen activator in cultured cells by macrocyclic plant diterpene esters and other agents related to tumor promotion.
    Cancer Res. 1978 May;38(5):1434-7 PMID: 639070
  17. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  18. Rapid stimulation of phospholipid metabolism in bovine lymphocytes by tumor-promoting phorbol esters.
    Cancer Res. 1978 Sep;38(9):2900-4 PMID: 679198
  19. Tumor-promoting phorbol esters inhibit binding of epidermal growth factor to cellular receptors.
    Science. 1978 Oct 20;202(4365):313-5 PMID: 308698
  20. An immunoradiometric assay for factor III (tissue thromboplastin).
    Br J Haematol. 1978 Jul;39(3):445-58 PMID: 698121
  21. Tissue thromboplastin activity of isolated human monocytes.
    Thromb Haemost. 1978 Jun 30;39(3):582-91 PMID: 705686
  22. Tumor promoters: effects on proliferation and differentiation of cells in culture.
    Life Sci. 1978 Nov 13;23(20):1979-88 PMID: 364230
  23. Cyclic adenosine 3', 5'-monophosphate and the mechanism of action of three common anti-inflammatory drugs.
    Br J Pharmacol. 1979 Feb;65(2):163-5 PMID: 216451
  24. Induction of differentiation in human promyelocytic leukemia cells by tumor promoters.
    Science. 1979 May 25;204(4395):868-70 PMID: 286421
  25. Changes in morphology and cell cycle traverse induced in CHO cells by methyl isobutyl xanthine.
    Exp Cell Res. 1979 Jul;121(2):383-94 PMID: 87332
  26. The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate enhances the proliferative response of Balb/c-3T3 cells to hormonal growth factors.
    J Cell Physiol. 1979 Sep;100(3):413-24 PMID: 314942
  27. Elimination of metabolic cooperation in Chinese hamster cells by a tumor promoter.
    Science. 1979 Nov 30;206(4422):1089-91 PMID: 493994
  28. In vitro stimulation of tissue thromboplastin (factor III) activity in human monocytes by immune complexes and lectins.
    Thromb Res. 1979;15(3-4):465-74 PMID: 494155
  29. Phorbol myristate acetate-induced release of granule enzymes from human neutrophils: inhibition by the calcium antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate hydrochloride.
    Biochem Biophys Res Commun. 1979 Nov 14;91(1):263-71 PMID: 518625
  30. Tumor promoters inhibit metabolic cooperation in cocultures of epidermal and 3T3 cells.
    Biochem Biophys Res Commun. 1979 Nov 28;91(2):395-401 PMID: 518638
  31. Cyclic nucleotide levels in rat embryo fibroblasts treated with tumor-promoting phorbol diester.
    J Cyclic Nucleotide Res. 1979 Oct;5(5):385-95 PMID: 230210
  32. Rapid alteration in Ca++ content and fluxes in phorbol 12-myristate 13-acetate treated myoblasts.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):624-30 PMID: 7356487
  33. Specific binding of phorbol ester tumor promoters.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):567-71 PMID: 6965793
  34. A radioimmunoassay for cyclic AMP (cAMP) obtained by acetylation of both unlabeled and labeled (3H-cAMP) ligand, or of unlabeled ligand only.
    Acta Pharmacol Toxicol (Copenh). 1980 Mar;46(3):200-4 PMID: 6244716
  35. A re-evaluation of the concept of cocarciongenesis.
    Prog Exp Tumor Res. 1969;11:21-30 PMID: 4891849
  36. Effects of xanthine derivatives on lipolysis and on adenosine 3',5'-monophosphate phosphodiesterase activity.
    Mol Pharmacol. 1970 Nov;6(6):597-603 PMID: 4322367
  37. Cytochemical identification of monocytes and granulocytes.
    Am J Clin Pathol. 1971 Mar;55(3):283-90 PMID: 5549896
  38. Factor X. Immunological studies.
    Thromb Diath Haemorrh. 1971;25(1):157-65 PMID: 4325293
  39. The inhibition of croton oil-promoted mouse skin tumorigenesis by steroid hormones.
    Cancer Res. 1972 Mar;32(3):450-4 PMID: 5061300
  40. Prophylaxis of chemically induced benign and malignant epithelial tumors by vitamin A acid (retinoic acid).
    Eur J Cancer. 1972 Dec;8(6):689-93 PMID: 4652000
  41. Calcium-dependent stimulation by a phorbol ester (PMA) of thymic lymphoblast DNA synthesis and proliferation.
    J Cell Physiol. 1973 Oct;82(2):151-6 PMID: 4753420
  42. The protein component of human brain thromboplastin.
    Biochem Biophys Res Commun. 1973 Dec 10;55(3):969-76 PMID: 4761090
  43. The tumor-promoter phorbol ester (12-O-tetradecanoyl-phorbol-13-acetate), a potent aggregating agent for blood platelets.
    J Cell Biol. 1974 Feb;60(2):325-36 PMID: 4360292
  44. Specific degranulation of human polymorphonuclear leukocytes.
    Nature. 1974 Mar 22;248(446):347-8 PMID: 4819643
  45. The function and mechanism of promoters of carcinogenesis.
    CRC Crit Rev Toxicol. 1974 Jan;2(4):419-43 PMID: 4822436
  46. Effect of some drugs on thromboplastin (factor III) activity of human monocytes in vitro.
    Biochem Pharmacol. 1980 Jan 1;29(1):9-14 PMID: 6244830
  47. Lectin stimulation of tissue thromboplastin activity in human monocytes in vitro.
    Thromb Haemost. 1980 Feb 29;42(5):1574-9 PMID: 7368158
  48. Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles.
    Biochem J. 1955 Mar;59(3):433-8 PMID: 14363113
  49. Growth stimulation of human diploid fibro-blasts by the tumor promoter, 12-0-tetradecanoly-phorbol-13-acetate.
    Int J Cancer. 1974 May 15;13(5):721-30 PMID: 4843882
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1940699
Published
1981-03-15
Pages
699-706
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1162804
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1940699 PMC Full Text View journal details
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