Home LiteratureArticle Details
PMID: 6273800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5601-7

Kato A, Shimada H, Kusuda M, Sugiura M

Abstract

Recombinant plasmids which contain EcoRI fragments of tobacco chloroplast DNA carrying tRNA genes were constructed. Plasmids pTC211 and pTC293 contain the base sequences for tRNAAsn in their 1.4 and 1.1 Md EcoRI fragments, respectively. These two tRNA sequences are identical and are; 5'-TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACCGATTGGTCGTAGGTTCGAATCCTACTTGGGGAG-3'. Each tRNAAsn gene is located at about 0.9 kb apart from the distal end of each 5S rRNA gene and is coded for by the DNA strand opposite from that of the rRNA genes.

MeSH Terms
Aspartate-tRNA Ligase Base Sequence Chloroplasts/metabolism DNA Restriction Enzymes DNA, Recombinant Genes Plants/metabolism Plants, Toxic Plasmids RNA, Transfer, Amino Acyl/genetics Tobacco/metabolism
Chemicals
DNA, Recombinant RNA, Transfer, Amino Acyl DNA Restriction Enzymes Aspartate-tRNA Ligase asparaginyl-tRNA synthetase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kato A
Shimada H
Kusuda M
Sugiura M
References (17)
17 references, click to expand
  1. DNA sequences of promoter regions for rRNA operons rrnE and rrnA in E. coli.
    Cell. 1979 May;17(1):201-9 PMID: 378405
  2. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.
    Nature. 1980 Aug 28;286(5776):908-10 PMID: 6774264
  3. Compilation of tRNA sequences.
    Nucleic Acids Res. 1981 Jan 10;9(1):r1-23 PMID: 7208351
  4. Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423-7 PMID: 16593030
  5. Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts.
    Gene. 1980 Jul;10(2):95-103 PMID: 6248424
  6. Hybridization of bean, spinach, maize and Euglena chloroplast transfer RNAs with homologous and heterologous chloroplast DNAs. An approach to the study of homology between chloroplast tRNAs from various species.
    Biochim Biophys Acta. 1980 Aug 26;609(1):31-9 PMID: 7407185
  7. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids. II. Comparison of the 5'-terminal nucleotide sequences of ribosomal RNA's from different organisms.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1595-602 PMID: 4296143
  8. Transfer ribonucleic acid genes in the chloroplast deoxyribonucleic acid of pea leaves.
    Biochemistry. 1980 Dec 23;19(26):5973-81 PMID: 6907019
  9. The transfer RNAs of eukaryotic organelles.
    Prog Nucleic Acid Res Mol Biol. 1978;21:143-79 PMID: 358277
  10. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  11. Purification of the T4 DNA ligase by Blue Sepharose chromatography.
    Anal Biochem. 1980 Nov 1;108(2):227-9 PMID: 7006454
  12. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA.
    Gene. 1979 Aug;6(4):285-306 PMID: 499808
  13. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  14. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  15. Mapping of rRNA genes in an inverted repeat in Nicotiana tabacum chloroplast DNA.
    Nucleic Acids Res. 1980 Aug 25;8(16):3505-16 PMID: 6253903
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions.
    J Biol Chem. 1980 Nov 25;255(22):10997-1003 PMID: 6253497
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.21.5601
Published
1981-11-11
Pages
5601-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327546
Subset
IM
Databases
GENBANK
J01451
External Links
PubMed source DOI: 10.1093/nar/9.21.5601 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2