Home LiteratureArticle Details
PMID: 6273804 Published · ppublish English Comparative Study Journal Article

Nucleotide sequence of the Aspergillus nidulans mitochondrial gene coding for the small ribosomal subunit RNA: homology to E. coli 16S rRNA.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5689-96

Köchel HG, Küntzel H

Abstract

The complete primary structure of the 1437 bp gene coding for mitochondrial 15S rRNA and its flanking regions was determined by Maxam-Gilbert sequencing of cloned HindIII fragment H3 of A. nidulans mtDNA. The gene product reveals significant homology (59%) to E. coli 16S rRNA, and the potential secondary structures of both rRNA molecules are very similar, except that the hairpin structures 7, 8 and 30 of the Brimacombe 16S rRNA model are deleted, and that two sequences of 8 and 31 nucleotides are inserted in the mitochondrial species.

MeSH Terms
Aspergillus nidulans/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Mitochondrial/genetics DNA, Recombinant Escherichia coli/genetics Genes RNA, Ribosomal/genetics Species Specificity
Chemicals
DNA, Mitochondrial DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Köchel H G
Küntzel H
References (14)
14 references, click to expand
  1. [Nucleotide sequence of the gene for the mitochondrial 15S ribosomal RNA of yeast].
    C R Seances Acad Sci D. 1980 Dec 8;291(12):933-6 PMID: 6261980
  2. Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
    Nature. 1981 May 21;291(5812):205-8 PMID: 7015146
  3. Secondary structure comparisons between small subunit ribosomal RNA molecules from six different species.
    Nucleic Acids Res. 1981 Aug 11;9(15):3621-40 PMID: 7024918
  4. The cloning of Aspergillus nidulans mitochondrial DNA in Escherichia coli on plasmid pBR322.
    Mol Gen Genet. 1981;182(2):332-5 PMID: 6270505
  5. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  6. Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans.
    Nucleic Acids Res. 1978 Feb;5(2):317-30 PMID: 345242
  7. Mitochondrial tRNA gene clusters in Aspergillus nidulans: organization and nucleotide sequence.
    Cell. 1981 Feb;23(2):625-33 PMID: 7008953
  8. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  9. Physical map of Aspergillus nidulans mitochondrial genes coding for ribosomal RNA: an intervening sequence in the large rRNA cistron.
    Mol Gen Genet. 1980 Feb;177(3):389-97 PMID: 6246396
  10. Distinctive sequence of human mitochondrial ribosomal RNA genes.
    Nature. 1980 Jul 31;286(5772):460-7 PMID: 6157106
  11. Precise localization and nucleotide sequence of the two mouse mitochondrial rRNA genes and three immediately adjacent novel tRNA genes.
    Cell. 1980 Nov;22(1 Pt 1):157-70 PMID: 7428037
  12. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
    Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
  13. The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Dec 11;8(23):5779-94 PMID: 7008030
  14. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1981-11-11
Pages
5689-96
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327553
Subset
IM
Databases
GENBANK
J01390
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]