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PMID: 6273805 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The high affinity binding site on polyoma virus DNA for the viral large-T protein.

Nucleic acids research ·Vol. 9 ·No. 21 ·1981-11-11 ·Pages 5697-710

Gaudray P, Tyndall C, Kamen R, Cuzin F

Abstract

In order to map the high affinity binding site for the viral large-T protein on polyoma virus DNA, we have developed an assay which does not require purified protein. It is based on the specific elution of the large-T ATPase activity from calf thymus DNA cellulose by recombinant DNA molecules including known sequences of the viral DNA. Using this assay, a high affinity binding site has been mapped on the early region side of the ori region. Binding requires the integrity of a sequence /AGAGGC/TTCC/AGAGGC/ (nucleotides 49 to 64 in the DNA sequence of the A2 strain). Similar repeats of a PuGPuGGC sequence within less than 20 bases are not found within the viral coding regions, but are strikingly common in the control regions of papovaviruses and other eukaryotic DNAs.

MeSH Terms
Adenosine Triphosphatases/metabolism Antigens, Polyomavirus Transforming Binding Sites Chromatography, Affinity Cloning, Molecular DNA Restriction Enzymes DNA, Viral/metabolism Genes, Viral Kinetics Polyomavirus/metabolism Viral Proteins/isolation & purification,metabolism
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral Viral Proteins DNA Restriction Enzymes Adenosine Triphosphatases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gaudray P
Tyndall C
Kamen R
Cuzin F
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.21.5697
Published
1981-11-11
Pages
5697-710
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327554
Subset
IM
Databases
GENBANK
J02288, J02290, J02291, J02292, K00932, K00997, K01041, K01071, K01072, V01117, V01147
External Links
PubMed source DOI: 10.1093/nar/9.21.5697 PMC Full Text View journal details
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