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PMID: 6273839 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Directionality and polarity in recA protein-promoted branch migration.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 6018-22

Cox MM, Lehman IR

Abstract

The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular phi X174 DNA molecules. Analysis of the reaction by electron microscopy confirms that D loops containing short heteroduplex regions are rapidly formed at the ends of the linear duplex, followed by a relatively slow branch migration that converts the D loops to nicked circular duplexes (RFII) and displaced linear single strands. Heteroduplex extension and displacement of the linear single strand are concerted. Heterologous sequences within the linear duplex halt branch migration and lead to the accumulation of D loops. Although D loops can be formed at either end of the linear duplex, recA protein-promoted branch migration proceeds uniquely in the 3' leads to 5' direction relative to the (--) strand of the linear duplex.

MeSH Terms

Bacterial Proteins/genetics,isolation & purification DNA Restriction Enzymes DNA, Single-Stranded/genetics DNA, Viral/genetics Escherichia coli/genetics Kinetics Microscopy, Electron Protein Conformation Rec A Recombinases

Chemicals

Bacterial Proteins DNA, Single-Stranded DNA, Viral Rec A Recombinases DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cox M M

Lehman I R

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.10.6018

Published

1981-10-00

Pages

6018-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC348968

Subset

Grants

NIGMS NIH HHS · GM-06196 · United States

External Links

PubMed source DOI: 10.1073/pnas.78.10.6018 PMC Full Text View journal details

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Directionality and polarity in recA protein-promoted branch migration.

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