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PMID: 6273858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence-specific contacts between the RNA polymerase of vesicular stomatitis virus and the leader RNA gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 10 ·1981-10-00 ·Pages 6191-5

Keene JD, Thornton BJ, Emerson SU

Abstract

Methylation-protection studies with nucleocapsids from vesicular stomatitis virus indicate that the viral polymerase (L and NS proteins) contacts the genomic RNA template in the middle of the leader gene, 16-30 nucleotides from the 3' terminus. The data suggest that the NS protein binds to the sequence (formula, see text) and may function as an initiator protein for transcription.

MeSH Terms

Animals Base Sequence Cell Line Cricetinae DNA-Directed RNA Polymerases/metabolism Genes, Viral Kidney Methylation RNA, Viral/genetics Vesicular stomatitis Indiana virus/genetics

Chemicals

RNA, Viral DNA-Directed RNA Polymerases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Keene J D

Thornton B J

Emerson S U

References (15)

15 references, click to expand

Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions.
J Virol. 1972 Aug;10(2):297-309 PMID: 4342247
Vesicular stomatitis virus defective interfering particle containing a muted internal leader RNA gene.
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2090-4 PMID: 6264463
Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus.
J Virol. 1975 Jun;15(6):1348-56 PMID: 167189
Order of transcription of genes of vesicular stomatitis virus.
Proc Natl Acad Sci U S A. 1976 Feb;73(2):442-6 PMID: 174107
Sequential transcription of the genes of vesicular stomatitis virus.
Proc Natl Acad Sci U S A. 1976 May;73(5):1504-8 PMID: 179088
A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro.
Cell. 1976 Jun;8(2):197-204 PMID: 183891
Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles.
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225-9 PMID: 210454
Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus.
J Virol. 1978 Sep;27(3):560-7 PMID: 212581
Direct chemical method for sequencing RNA.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 PMID: 377283
Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus.
Cell. 1980 Feb;19(2):415-21 PMID: 6244108
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
Cell. 1980 Mar;19(3):717-28 PMID: 6153931
E. coli RNA polymerase interacts homologously with two different promoters.
Cell. 1980 Jun;20(2):269-81 PMID: 6248238
Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA.
J Virol. 1980 Feb;33(2):789-94 PMID: 6251249
Chemical probes for higher-order structure in RNA.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4679-82 PMID: 6159633
Analysis of the RNA of defective VSV particles.
Cell. 1974 Sep;3(1):85-93 PMID: 4369780

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.10.6191

Published

1981-10-00

Pages

6191-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349004

Subset

Grants

NIAID NIH HHS · AI11722 · United States

NIAID NIH HHS · R01 AI16099 · United States

External Links

PubMed source DOI: 10.1073/pnas.78.10.6191 PMC Full Text View journal details

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Sequence-specific contacts between the RNA polymerase of vesicular stomatitis virus and the leader RNA gene.

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