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PMID: 6273918 Published · ppublish English Journal Article

Diazepam and (--)-pentobarbital: fluctuation analysis reveals different mechanisms for potentiation of gamma-aminobutyric acid responses in cultured central neurons.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 7180-4

Study RE, Barker JL

Abstract

Diazepam and (--)-pentobarbital each potentiate the increase in chloride ion conductance produced by gamma-aminobutyric acid (GABA) i voltage-clamped mouse spinal neurons grown in culture. Fluctuation analysis was used to compare the properties of elementary ion-channel events underlying the chloride conductance produced by GABA alone and during potentiation by the two drugs. Neither drug altered the conductance of an open ion channel, but both drugs affected the kinetics of channel activity. Diazepam increased the frequency of channel openings and either did not affect or slightly increased the average open-channel lifetime, whereas (--)-pentobarbital decreased the frequency of channel openings and increased average open-channel lifetime. These changes in the kinetics of GABA-activated ion channels can quantitatively account for the potentiation of GABA responses observed with the drugs. Thus, the drugs each increase the response to GABA but do not act on channel kinetics in the same manner.

MeSH Terms
Animals Cells, Cultured Diazepam/pharmacology Embryo, Mammalian Ion Channels/physiology Membrane Potentials/drug effects Mice Mice, Inbred C57BL Neurons/drug effects,physiology Pentobarbital/pharmacology Spinal Cord/physiology gamma-Aminobutyric Acid/pharmacology
Chemicals
Ion Channels gamma-Aminobutyric Acid Pentobarbital Diazepam
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Study R E
Barker J L
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.11.7180
Published
1981-11-00
Pages
7180-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349220
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.11.7180 PMC Full Text View journal details
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