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PMID: 6275096 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Integration of Rous sarcoma virus DNA into chicken embryo fibroblasts: no preferred proviral acceptor site in the DNA of clones of singly infected transformed chicken cells.

Journal of virology ·Vol. 40 ·No. 2 ·1981-11-00 ·Pages 421-30

Lerner TL, Skalka AM, Hanafusa H

Abstract

We analyzed retroviral integration into a host genome by using avian sarcoma virus infection of natural target cells under conditions where secondary integration via virus spread was inhibited. This was accomplished by using the noninfectious pol- env- alpha variant of the Bryan high-titer strain of Rous sarcoma virus. A total of 12 independent Bryan high-titer Rous sarcoma virus-transformed chicken embryo fibroblast clones were obtained and mapped by using restriction endonucleases. Provirus-cell junction fragments were identified with appropriate hybridization probes. We found that expression of the viral genes could occur after proviral integration at many sites on the chicken genome and that there was no apparent preference for specific integration sites.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cell Transformation, Viral Chick Embryo/microbiology Clone Cells/microbiology DNA Restriction Enzymes DNA, Viral/genetics Fibroblasts Recombination, Genetic
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lerner T L
Skalka A M
Hanafusa H
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47 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.2.421-430.1981
Published
1981-11-00
Pages
421-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256643
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
External Links
PubMed source DOI: 10.1128/JVI.40.2.421-430.1981 PMC Full Text View journal details
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