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PMID: 6275110 Published · ppublish English Journal Article

Mutant carrying deletions in the two simian virus 40 early genes.

Journal of virology ·Vol. 40 ·No. 3 ·1981-12-00 ·Pages 625-34

Feunteun J, Carmichael G, Nicolas JC, Kress M

Abstract

We isolated a simian virus 40 mutant, dl2194, which carried deletions in both early genes. One deletion removed 234 base pairs in the 54/59 region within the small-t-antigen coding sequence and the large-T-antigen gene intron. The second deletion removed 57 base pairs at the C terminus of the large-T-antigen coding sequence (0.20 map unit). dl2194 was a viable mutant, it carried a normal helper function for adenovirus growth on monkey cells, and it displayed the transformation properties of a small-t-antigen-negative single mutant. Therefore, none of the known large-T-antigen functions seemed to be altered by the C-terminal deletion.

MeSH Terms
Adenoviruses, Human/growth & development Animals Antigens, Neoplasm/genetics Antigens, Viral/genetics Antigens, Viral, Tumor Base Sequence Cell Line Cell Transformation, Viral DNA, Viral Genes, Viral Haplorhini Helper Viruses/physiology Humans Mutation Simian virus 40/genetics,physiology
Chemicals
Antigens, Neoplasm Antigens, Viral Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Feunteun J
Carmichael G
Nicolas J C
Kress M
References (31)
31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.3.625-634.1981
Published
1981-12-00
Pages
625-34
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256674
Subset
IM
Databases
GENBANK
J02400, J02402, J02403, J02406, J02407, J02408, J02409, J02410, V01380
External Links
PubMed source DOI: 10.1128/JVI.40.3.625-634.1981 PMC Full Text View journal details
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