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PMID: 6275132 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning overlapping DNA fragments from the B95-8 strain of Epstein-Barr virus reveals a site of homology to the internal repetition.

Journal of virology ·Vol. 40 ·No. 3 ·1981-12-00 ·Pages 977-82

Buell GN, Reisman D, Kintner C, Crouse G, Sugden B

Abstract

Overlapping, sheared DNA fragments from the B95-8 strain of Epstein-Barr virus were cloned in Charon 4A. Eleven recombinant phages plus one recombinant plasmid contained all of the sequences found in B95-8 virion DNA. Analysis of recombinant DNA molecules revealed a previously undetected site of homology to the internal repetition found in Epstein-Barr virus DNA. This site was adjacent to or at a site which was unstable when the recombinant DNA was propagated as phage DNA in procaryotic hosts.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant DNA, Viral Deoxyribonuclease EcoRI Deoxyribonuclease HindIII Herpesvirus 4, Human/genetics Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant DNA, Viral DNA Restriction Enzymes Deoxyribonuclease EcoRI Deoxyribonuclease HindIII
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Buell G N
Reisman D
Kintner C
Crouse G
Sugden B
References (12)
12 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.3.977-982.1981
Published
1981-12-00
Pages
977-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256713
Subset
IM
Grants
NCI NIH HHS · CA-09135 · United States
NCI NIH HHS · CA-22443 · United States
NCI NIH HHS · CA-97175 · United States
External Links
PubMed source DOI: 10.1128/JVI.40.3.977-982.1981 PMC Full Text View journal details
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