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PMID: 6276670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal loci of genes controlling site-specific restriction endonucleases of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 183 ·No. 1 ·1981-00-00 ·Pages 1-6

Ikawa S, Shibata T, Matsumoto K, Iijima T, Saito H, Ando T

Abstract

We constructed transformation of B. subtilis 168 which acquired genes for site-specific restriction endonucleases. These endonucleases originated from various strains of B. subtilis and were classified into five groups based on the specificity of the sequences recognized by the enzymes. We examined the loci of genes for site-specific restriction endonucleases belonging to different groups: hsrE determined Endo. R. Bsu1231 (I), hsrB Endo.R.Bsu1247(I), hsrR Endo.R.BsuR and hsrC Endo.R.Bsu-1247(II). One gene, hsrE, was located between sacA and purA by transduction crosses with phage PBS1, and another gene, hsrB, between hsrE and purA. Genes hsrR and hsrC had been suggested to be allelic or closely linked by previous studies with transformation. We located hsrR and hsrC between purB and tre. Our previous observation and this study show that B. subtilis 168 has at least three independent loci on the chromosome for four genes for site-specific restriction endonucleases in addition to the locus for the original restriction activity (Bsu168-specific restriction) of strain 168.

MeSH Terms
Bacillus subtilis/genetics Chromosome Mapping DNA Restriction Enzymes/genetics Genes Genotype Phenotype Transformation, Bacterial
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ikawa S
Shibata T
Matsumoto K
Iijima T
Saito H
Ando T
References (33)
33 references, click to expand
  1. Identification of the structural gene of levansucrase in Bacillus subtilis Marburg.
    Mol Gen Genet. 1974 Feb 6;128(3):213-21 PMID: 4209418
  2. The site-specific deoxyribonuclease from Bacillus pumilus (endonuclease R.Bpu1387).
    J Biochem. 1976 Dec;80(6):1457-60 PMID: 1018024
  3. Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria.
    Virology. 1957 Jun;3(3):496-513 PMID: 13443211
  4. Phenotypically cryptic EcoRI endonuclease activity specified by the ColE1 plasmid.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1265-9 PMID: 349563
  5. Host controlled modification and restriction in Bacillus subtilis.
    Mol Gen Genet. 1974;131(4):275-80 PMID: 4215952
  6. In vivo site-specific genetic recombination promoted by the EcoRI restriction endonuclease.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4811-5 PMID: 337302
  7. Transformation and transduction in Bacillus subtilis: evidence for separate modes of recombinant formation.
    J Mol Biol. 1969 Oct 28;45(2):155-79 PMID: 4983717
  8. Genetic mapping in Bacillus subtilis.
    J Mol Biol. 1967 Jul 14;27(1):163-85 PMID: 4962145
  9. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.
    J Mol Biol. 1966 Mar;16(1):118-33 PMID: 5331240
  10. Restriction and modification of bacteriophage in Bacillus stearothermophilus.
    J Virol. 1973 Apr;11(4):606-9 PMID: 4700514
  11. Site-specific deoxyribonucleases in Bacillus subtilis and other Bacillus strains.
    J Bacteriol. 1976 Oct;128(1):473-6 PMID: 824277
  12. Recognition sequence of a restriction enzyme.
    Nat New Biol. 1973 Jul 4;244(131):7-10 PMID: 4578426
  13. Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.
    Mol Gen Genet. 1975 Dec 30;143(1):25-33 PMID: 814403
  14. The Bacillus subtilis chromosome.
    Microbiol Rev. 1980 Mar;44(1):57-82 PMID: 6774224
  15. Genetic mapping of a defective bacteriophage on the chromosome of Bacillus subtilis 168.
    J Virol. 1970 Sep;6(3):340-3 PMID: 4991424
  16. Restriction and modification enzymes and their recognition sequences.
    Gene. 1980 Mar;8(4):329-43 PMID: 6245015
  17. Bacillus subtilis bacteriophage SPbeta: localization of the prophage attachment site, and specialized transduction.
    J Bacteriol. 1977 Jan;129(1):556-8 PMID: 401505
  18. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
    J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
  19. The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificities.
    Biochim Biophys Acta. 1976 Aug 18;442(2):184-96 PMID: 182257
  20. Introduction of host-controlled modification and restriction systems of Bacillus subtilis IAM1247 into Bacillus subtilis 168.
    J Bacteriol. 1979 Jul;139(1):308-10 PMID: 110786
  21. Repair of ultraviolet-induced DNA damage in the subcellular systems of Bacillus subtilis.
    Mutat Res. 1973 Nov;20(2):159-73 PMID: 4201874
  22. In vitro modification and restriction of phage phi-105c DNA with Bacillus subtilis N cell-free extract.
    Mol Gen Genet. 1975 Jul 10;138(4):269-79 PMID: 807794
  23. Isolation and characterization of prophage mutants of the defective Bacillus subtilis bacteriophage PBSX.
    J Virol. 1975 Jul;16(1):184-91 PMID: 805847
  24. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.
    J Mol Biol. 1975 Sep 5;97(1):123-5 PMID: 1177312
  25. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome.
    J Bacteriol. 1975 Mar;121(3):823-34 PMID: 803963
  26. INDUCIBLE PHAGES OF BACILLUS SUBTILIS.
    Biochemistry. 1964 May;3:607-13 PMID: 14193626
  27. Restriction and modification in B. subtilis. Biological aspects.
    Mol Gen Genet. 1974;131(3):181-91 PMID: 4215951
  28. Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg.
    Mol Gen Genet. 1979 Feb 26;170(2):117-22 PMID: 107390
  29. Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.
    Mol Gen Genet. 1975 Dec 30;143(1):13-23 PMID: 2856
  30. A restriction enzyme from Hemophilus influenzae. II.
    J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
  31. Host-controlled modification and restriction in Bacillus subtilis: Bsu 168-system and BsuR-system in B. subtilis 168.
    Mol Gen Genet. 1979 Feb 26;170(2):123-7 PMID: 107391
  32. Genetic studies on site-specific endodeoxyribonucleases in Bacillus subtilis: multiple modification and restriction systems in transformants of Bacillus subtilis 168.
    Mol Gen Genet. 1980 Feb;177(3):359-68 PMID: 6246395
  33. A restriction endonuclease analysis of the bacterial plasmid controlling the ecoRI restriction and modification of DNA.
    Fed Proc. 1976 Jul;35(9):2037-43 PMID: 776705
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270129
Published
1981-00-00
Pages
1-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270129
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