P1 transduction map spanning the replication terminus of Escherichia coli K12.
The region of the E. coli chromosome that contains the replication terminus has not previously been spanned by P1 cotransduction. We have used Tn5, Tn9 and Tn10 transposons inserted in this region as genetic markers, and have constructed a genetic map that extends from fnr (min 29.3) to manA (min 35.7). The relevant transposons that have been mapped in this region and which are described in this report are trg-1::Tn5 (min 31.1), zdc-235::Tn10 (min 32.3), zdd-230::Tn9 (min 33.3), and zde-234::Tn10 (min 34.2). The size of this region as determined by P1 cotransduction is very similar to previous estimates obtained by bacterial conjugation.
19 references, click to expand
-
Chromosome replication in Escherichia coli is inhibited in the terminus region near the rac locus.Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:563-7 PMID: 383383
-
Regulation of Tn5 transposition in Salmonella typhimurium.Proc Natl Acad Sci U S A. 1980 Oct;77(10):6047-51 PMID: 6255475
-
Evidence for a fixed termination site of chromosome replication in Escherichia coli K12.J Mol Biol. 1977 Sep 25;115(3):295-314 PMID: 338909
-
Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.J Bacteriol. 1981 Feb;145(2):914-9 PMID: 6257656
-
Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda.Genetics. 1978 Nov;90(3):427-61 PMID: 365678
-
Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene.J Bacteriol. 1977 Jul;131(1):49-56 PMID: 326769
-
Linkage map of Escherichia coli K-12, edition 6.Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
-
Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.Mol Gen Genet. 1977 Sep 9;154(3):305-9 PMID: 337110
-
Terminus region of the chromosome in Escherichia coli inhibits replication forks.Proc Natl Acad Sci U S A. 1977 Sep;74(9):3927-31 PMID: 333449
-
Mutants of Escherichia coli K12 unable to use fumarate as an anaerobic electron acceptor.J Gen Microbiol. 1976 Dec;97(2):145-60 PMID: 796407
-
Mutants in transmission of chemotactic signals from two independent receptors of E. coli.Cell. 1979 Mar;16(3):617-25 PMID: 378395
-
Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis.Mol Gen Genet. 1979 Mar 20;171(2):193-203 PMID: 375029
-
Map position of the replication terminus on the Escherichia coli chromosome.Mol Gen Genet. 1979 Apr 17;172(1):7-11 PMID: 377025
-
P1 transduction mapping of the trg locus in rac+ and rac strains of Escherichia coli K-12.J Bacteriol. 1982 Feb;149(2):529-33 PMID: 6276359
-
A model for three-point analysis of random general transduction.Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
-
Recalibrated linkage map of Escherichia coli K-12.Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
-
Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
-
A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit.Proc Natl Acad Sci U S A. 1974 Sep;71(9):3465-9 PMID: 4610577
-
Isolation and properties of Tn10 insertions in the rac locus of Escherichia coli.Mol Gen Genet. 1981;183(2):333-40 PMID: 6276684
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
