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PMID: 6278251 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A fine structure map of spontaneous and induced mutations in the lambda repressor gene, including insertions of IS elements.

Molecular & general genetics : MGG ·Vol. 184 ·No. 3 ·1981-00-00 ·Pages 364-71

Lieb M

Abstract

Mutations at over 70 sites in the cI gene have been mapped by 4-factor crosses and assigned precise or approximate positions in the DNA sequence. 16 of 25 spontaneous mutations were insertions of IS1, IS3 or IS5 into AT-rich regions of cI. The 5-methylcytosine in the sequence Cm5CAGG is a hot spot for spontaneous cI amber mutations. Recombination frequencies between mutations were proportional to distance with the exception of amber mutations at 4 sites, including the host spot for spontaneous mutations. Mutations with a given phenotype are clustered on the genetic map. No missense mutations affecting repressor activity were found in the central one-third of cI, but 5 of 6 ind- mutations were located in this region. The amino-terminal third of the gene contains the sites of most trans-dominant cI- mutations, and of all ts mutations that result in repressors that are reversibly inactivated at high temperatures.

MeSH Terms
Bacteriophage lambda/genetics DNA Transposable Elements Escherichia coli/genetics Genes Genotype Mutation Repressor Proteins/genetics Species Specificity Transcription Factors/genetics
Chemicals
DNA Transposable Elements Repressor Proteins Transcription Factors
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lieb M
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53 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00352506
Published
1981-00-00
Pages
364-71
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM25034 · United States
External Links
PubMed source DOI: 10.1007/BF00352506
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