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PMID: 6278426 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Secondary structure of Bombyx mori and Dictyostelium discoideum 5S rRNA from S1 nuclease and cobra venom ribonuclease susceptibility, and computer assisted analysis.

Nucleic acids research ·Vol. 10 ·No. 2 ·1982-01-22 ·Pages 653-64

Troutt A, Savin TJ, Curtiss WC, Celentano J, Vournakis JN

Abstract

The 5S rRNAs from Bombyx mori and Dictyostelium discoideum were end-labeled with [32-P] at either the 5' or 3' end and sequenced using enzymatic digestion. The secondary structure of these molecules was studied using the single-strand specific S1 nuclease and the base-pair specific cobra venom ribonuclease. Computer analysis of these results was performed and was used to generate a consensus secondary structure for each molecule. A comparison of these results with those of other workers is presented.

MeSH Terms
Animals Aspergillus oryzae/enzymology Base Sequence Bombyx/analysis Computers Dictyostelium/analysis Elapid Venoms Endonucleases Nucleic Acid Conformation RNA, Ribosomal Ribonucleases Single-Strand Specific DNA and RNA Endonucleases Species Specificity
Chemicals
Elapid Venoms RNA, Ribosomal Endonucleases Ribonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Troutt A
Savin T J
Curtiss W C
Celentano J
Vournakis J N
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.2.653
Published
1982-01-22
Pages
653-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326164
Subset
IM
Grants
NIGMS NIH HHS · GM22280 · United States
External Links
PubMed source DOI: 10.1093/nar/10.2.653 PMC Full Text View journal details
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