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PMID: 6278440 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A lac promoter with a changed distance between -10 and -35 regions.

Nucleic acids research ·Vol. 10 ·No. 3 ·1982-02-11 ·Pages 903-12

Mandecki W, Reznikoff WS

Abstract

A lac promotor mutant was constructed by filling in the protruding ends of the HpaII site located within the lac promotor. The mutation, named M42, is a two base pair insertion that changes the distance between the -10 and -35 regions from 18 to 20 residues. The activity of the mutant promotor measured in vivo is 15% of the wild-type promotor. The M42 promotor is sensitive to the catabolite repression in the manner similar to that of the wild type. Sequences of several deletions within the lac promotor are also given.

MeSH Terms
Base Sequence DNA Restriction Enzymes Escherichia coli/genetics Lac Operon Mutation Plasmids
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mandecki W
Reznikoff W S
References (10)
10 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.3.903
Published
1982-02-11
Pages
903-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326210
Subset
IM
Grants
NIGMS NIH HHS · R01-GM19670 · United States
External Links
PubMed source DOI: 10.1093/nar/10.3.903 PMC Full Text View journal details
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