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PMID: 6279255 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alkaline phosphatase inhibition by parathyroid hormone and isoproterenol in a clonal rat osteosarcoma cell line. Possible mediation by cyclic AMP.

Calcified tissue international ·Vol. 34 ·No. 1 ·1982-01-00 ·Pages 59-66

Majeska RJ, Rodan GA

Abstract

The effect of parathyroid hormone (PTH 1-34 bovine) on alkaline phosphatase activity was investigated in an osteoblast-like clonal cell line derived from rat osteosarcoma (ROS 17/2). ROS 17/2 alkaline phosphatase resembled the bone enzyme in levamisole sensitivity and electrophoretic mobility but differed in heat stability. The specific activity of ROS 17/2 alkaline phosphatase increased with time in culture. This increase was inhibited by PTH (1-34) and (-)-isoproterenol in a dose-dependent manner starting at near-physiological hormone concentrations. The ID50 values were 0.02 nM for PTH (1-34) and 1.7 nM for isoproterenol. The two hormones stimulated ROS 17/2 adenylate cyclase, albeit at higher concentrations: Km values were 13 nM for PTH (1-34) and 16 nM for isoproterenol. The rise in alkaline phosphatase was also inhibited by dibutyryl cyclic AMP and 8-bromocyclic AMP (0.1 mM). These findings further document the osteoblastic properties of the ROS 17/2 osteosarcoma cell line, suggest that PTH inhibition of alkaline phosphatase represents a physiological response to the hormone in these cells, and implicate cyclic AMP as a mediator of this PTH effect.

MeSH Terms
Alkaline Phosphatase/antagonists & inhibitors Animals Bone Neoplasms/enzymology Bucladesine/pharmacology Cell Line Cyclic AMP/physiology Electrophoresis, Polyacrylamide Gel Enzyme Induction/drug effects Isoproterenol/pharmacology Osteosarcoma/enzymology Parathyroid Hormone/physiology Rats
Chemicals
Parathyroid Hormone Bucladesine Cyclic AMP Alkaline Phosphatase Isoproterenol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Majeska R J
Rodan G A
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39 references, click to expand
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Article Info
Journal
Calcified tissue international
Abbr.
Calcif Tissue Int
ISSN
0171-967X
DOI
10.1007/BF02411210
Published
1982-01-00
Pages
59-66
Language
English
Region
United States
NLM ID
7905481
Subset
IM
Grants
NIADDK NIH HHS · AM-17848 · United States
NIDCR NIH HHS · DE-04327 · United States
External Links
PubMed source DOI: 10.1007/BF02411210 View journal details
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