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PMID: 6280135 Published · ppublish English Journal Article

Characterization of sequence elements at the 5' end of a discoidin I gene isolated from Dictyostelium discoideum.

Nucleic acids research ·Vol. 10 ·No. 4 ·1982-02-25 ·Pages 1231-41

Devine JM, Williams JG

Abstract

The discoidin I genes of Dictyostelium discoideum encode a family of three closely related developmentally regulated lectins which may play a role in cell cohesion. We have isolated a genomic clone containing the 5' half of a discoidin I gene with 12kb of flanking sequence and we have identified the discoidin I polypeptide encoded by this gene. We have determined part of the nucleotide sequence of the cloned segment and have hence determined the N-terminal amino acid sequence of the encoded polypeptide. The nucleotide sequence upstream of the AUG initiation codon was unusually AT rich, with 94% AT base pairs in the 171 nucleotides for which sequence was determined. However, having determined the start point of transcription ('cap' site) two striking features of similarity with other eukaryotic structural genes became apparent. The sequence TATAAA was present 33 nucleotides upstream from the 'cap' site and the sequence CCAAT was present a further 20 nucleotides upstream.

MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Fungal/genetics Dictyostelium/genetics Discoidins Fungal Proteins/genetics Genes Lectins Protozoan Proteins
Chemicals
Carrier Proteins DNA, Fungal Discoidins Fungal Proteins Lectins Protozoan Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Devine J M
Williams J G
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.4.1231
Published
1982-02-25
Pages
1231-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320521
Subset
IM
Databases
GENBANK
J01284
External Links
PubMed source DOI: 10.1093/nar/10.4.1231 PMC Full Text View journal details
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