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PMID: 6280152 Published · ppublish English Comparative Study Journal Article

Physical and gene mapping of chloroplast DNA from Atriplex triangularis and Cucumis sativa.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1593-605

Palmer JD

Abstract

A rapid and simple method for constructing restriction maps of large DNAs (100-200 kb) is presented. The utility of this method is illustrated by mapping the Sal I, Sac I, and Hpa I sites of the 152 kb Atriplex triangularis chloroplast genome, and the Sal I and Pvu II sites of the 155 kb Cucumis sativa chloroplast genome. These two chloroplast DNAs are very similar in organization; both feature the near-universal chloroplast DNA inverted repeat sequence of 22-25 kb. The positions of four different genes have been localized on these chloroplast DNAs. In both genomes the 16S and 23S ribosomal RNAs are encoded by duplicate genes situated at one end of the inverted repeat, while genes for the large subunit of ribulose-1,5-bisphosphate carboxylase and a 32 kilodalton photosystem II polypeptide are separated by 55 kb of DNA within the large single copy region. The physical and genetic organization of these DNAs is compared to that of spinach chloroplast DNA.

MeSH Terms
Base Composition Base Sequence Chloroplasts/analysis DNA/genetics DNA Restriction Enzymes Genes Nucleic Acid Hybridization Plants/analysis Species Specificity
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Palmer J D
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20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1593
Published
1982-03-11
Pages
1593-605
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320552
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.5.1593 PMC Full Text View journal details
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