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PMID: 6281170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Assigning the polymorphic human insulin gene to the short arm of chromosome 11 by chromosome sorting.

Human genetics ·Vol. 60 ·No. 1 ·1982-00-00 ·Pages 10-15

Lebo RV, Kan YW, Cheung MC, Carrano AV, Yu LC, Chang JC, Cordell B, Goodman HM

Abstract

We have determined the subchromosomal location of the human insulin gene by analyzing DNA isolated from sorted human metaphase chromosomes. Metaphase chromosome suspensions were sorted into fractions according to relative Hoechst fluorescence intensity by the fluorescence activated chromosome sorter. The chromosomal DNA in each fraction was characterized by restriction endonuclease analysis. Initial sorts indicated that the insulin gene-containing fragment resided in a fraction containing chromosomes 9, 10, 11, and 12. Studies of cell lines that contained chromosome translocations permitted the assignment of the insulin gene to a derivative chromosome that contains portions of the short arm of chromosome II. Simultaneous sorting of the normal homolog from this small derivative chromosome separated the two different sized insulin gene-containing restriction fragments in this individual. These data indicate that the two restriction fragments represent insulin gene polymorphism and not duplicate gene loci.

MeSH Terms
Alleles Cell Line Cell Separation Chromosome Mapping Chromosomes, Human, 6-12 and X DNA/genetics DNA Restriction Enzymes Flow Cytometry Genes Humans Insulin/genetics Polymorphism, Genetic
Chemicals
Insulin DNA DNA Restriction Enzymes
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lebo R V
Kan Y W
Cheung M C
Carrano A V
Yu L C
Chang J C
Cordell B
Goodman H M
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27 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00281255
Published
1982-00-00
Pages
10-15
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
Grants
NIADDK NIH HHS · AM 16666 · United States
NCI NIH HHS · CA 14026 · United States
External Links
PubMed source DOI: 10.1007/BF00281255 View journal details
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