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PMID: 6281757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytosolic malic dehydrogenase activity is associated with a putative substrate for the transforming gene product of Rous sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 228-32

Rübsamen H, Saltenberger K, Friis RR, Eigenbrodt E

Abstract

A cellular protein of apparent Mr 34,000--36,000 was suggested as a possible physiological substrate for the protein kinase (EC 2.7.1.37) activity associated with the transforming gene product of Rous sarcoma virus. We find this protein to migrate with an apparent Mr of 38,000 in NaDodSO4/polyacrylamide gels. It was not separable from cytosolic malic dehydrogenase activity when purified by chromatography on DEAE-Sephacel, hydroxylapatite, poly(A)-Sepharose, and blue Sepharose, by gel filtration, and by isoelectric focusing. The Mr 38,000 protein as well as cytosolic malic dehydrogenase activity focused with a pI of 7.5. In gel filtration experiments, both displayed an apparent native Mr of 68,000. The male dehydrogenase activity contained in homogeneous preparations of the Mr 38,000 protein had a specific activity of up to 130 units/mg of protein. The recovery of the enzyme was 5--10% of the activity in the extract. Antiserum against the Mr 38,000 protein inactivated the malic dehydrogenase activity associated with the Mr 38,000 protein.

MeSH Terms
Animals Avian Sarcoma Viruses Cell Transformation, Viral Cells, Cultured Cytosol/enzymology Isoelectric Point Isoenzymes/metabolism Malate Dehydrogenase/metabolism Molecular Weight Oncogene Protein pp60(v-src) Phosphorylation Protein Kinases/metabolism Viral Proteins/metabolism
Chemicals
Isoenzymes Viral Proteins Malate Dehydrogenase Protein Kinases Oncogene Protein pp60(v-src)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rübsamen H
Saltenberger K
Friis R R
Eigenbrodt E
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.2.228
Published
1982-01-00
Pages
228-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345699
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.2.228 PMC Full Text View journal details
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