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PMID: 6281775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical map of the white locus of Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 2 ·1982-01-00 ·Pages 564-8

Levis R, Bingham PM, Rubin GM

Abstract

The white locus of Drosophila melanogaster is a genetically well-characterized locus, mutations in which alter the degree of pattern of pigmentation of the eyes. Using a previously cloned DNA segment containing a portion of the white locus of a mutant allele, we have cloned and characterized the DNA of a 48-kilobase chromosomal region of the Canton S wild-type strain. We have mapped the positions, relative to restriction endonuclease cleavage sites, of several previously characterized chromosomal rearrangement breakpoints that bracket the while locus. These results define a segment of 14 kilobase that contains all of the white locus sequences necessary for the production of a wild-type eye color phenotype. By conventional criteria, no repetitive sequences are present within this 14-kilobase segment; however, we have identified an extremely weak DNA sequence homology between a portion of this segment and a chromosomal region in the vicinity of the zeste locus.

MeSH Terms

Animals Bacteriophage lambda Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Drosophila melanogaster/genetics Eye Nucleic Acid Hybridization Pigmentation

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Levis R

Bingham P M

Rubin G M

References (23)

23 references, click to expand

Insertion of the Drosophila transposable element copia generates a 5 base pair duplication.
Cell. 1980 Sep;21(2):575-9 PMID: 6250725
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method.
Cell. 1981 Sep;25(3):693-704 PMID: 6269753
Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila.
Cell. 1979 Jun;17(2):415-27 PMID: 110463
Cytogenetic fine structure and chromosomal localization of the white gene in Drosophila melanogaster.
Nat New Biol. 1973 Sep 12;245(141):34-7 PMID: 4125726
Genetic instability in Drosophila melanogaster: evidence for regulation, excision and transposition at the white locus.
Mol Gen Genet. 1980;177(4):567-70 PMID: 6247608
The isolation of structural genes from libraries of eucaryotic DNA.
Cell. 1978 Oct;15(2):687-701 PMID: 719759
Genetic modulation of RNA metabolism in Drosophila. III. Requirement for an rDNA-deficient X chromosome in YbbSuVar-3-mediated increases in RNA synthesis.
Genetics. 1979 Oct;93(2):411-22 PMID: 119671
Allelic pairing and gene regulation: A model for the zeste-white interaction in Drosophila melanogaster.
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1368-72 PMID: 16592632
Restriction maps for twenty-one Charon vector phages.
J Virol. 1980 Jan;33(1):401-10 PMID: 6245241
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
SEX LIMITED INHERITANCE IN DROSOPHILA.
Science. 1910 Jul 22;32(812):120-2 PMID: 17759620
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
A novel dominant mutant allele at the white locus of Drosophila melanogaster is mutable.
Cold Spring Harb Symp Quant Biol. 1981;45 Pt 2:519-25 PMID: 6266745
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
Virology. 1970 Mar;40(3):734-44 PMID: 4908735
Molecular limits on the size of a genetic locus in Drosophila melanogaster.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7367-71 PMID: 6784122
Repetitive sequences in isolated Thomas circles from Drosophila melanogaster.
Cold Spring Harb Symp Quant Biol. 1974;38:371-81 PMID: 4208788
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
A copy of the copia transposable element is very tightly linked to the Wa allele at the white locus of D. melanogaster.
Cell. 1981 Sep;25(3):705-11 PMID: 6269754
The Regulation of White Locus Expression: A Dominant Mutant Allele at the White Locus of DROSOPHILA MELANOGASTER.
Genetics. 1980 Jun;95(2):341-53 PMID: 17249039
Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster.
Cell. 1980 Apr;19(4):897-904 PMID: 6247074
Drosophila genome organization: conserved and dynamic aspects.
Annu Rev Genet. 1981;15:219-64 PMID: 6802066

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.2.564

Published

1982-01-00

Pages

564-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC345785

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.2.564 PMC Full Text View journal details

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Physical map of the white locus of Drosophila melanogaster.

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