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PMID: 6283312 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A DNA sequence containing the control regions of the malEFG and malK-lamB operons in Escherichia coli K12.

Molecular & general genetics : MGG ·Vol. 185 ·No. 1 ·1982-00-00 ·Pages 82-7

Bedouelle H, Hofnung M

Abstract

The malB region in E. coli is composed of two operons, malEFG and malK-lamB, transcribed divergently from a control region located between the malE and malK genes. We have established the DNA sequence of a 600 base pair segment which contains the sites necessary for initiation of transcription of the malB operons and the translation start sites for malE and malK. We have also determined the position of a 147 base pair deletion, malB delta 100, in this sequence. The control region contains two short segments (about 35 base pairs) showing unusual distributions of bases: the GC content (85%) is higher than average and most of the purines (85%) are on one strand. We discuss the possible relevance of this and other salient features of the DNA sequence to the mechanisms by which the expression of the malB region is positively regulated.

MeSH Terms
Base Composition Base Sequence Chromosomes, Bacterial/ultrastructure DNA Restriction Enzymes DNA, Bacterial/analysis Escherichia coli/ultrastructure Gene Expression Regulation Genes Operon Protein Biosynthesis Purine Nucleotides/metabolism
Chemicals
DNA, Bacterial Purine Nucleotides DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bedouelle H
Hofnung M
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39 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333794
Published
1982-00-00
Pages
82-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
J01639, J01648, K02117
External Links
PubMed source DOI: 10.1007/BF00333794
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