Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2177-87

Harris TJ, Lowe PA, Lyons A, Thomas PG, Eaton MA, Millican TA, Patel TP, Bose CC, Carey NH, Doel MT

Abstract

DNA complementary to calf stomach mRNA has been synthesised and inserted into the Pst1 site of pAT153 by G-C tailing. Clones containing sequences coding for prochymosin were recognised by colony hybridisation with cDNA extended from a chemically synthesised oligodeoxynucleotide primer, the sequence of which was predicted from the published amino acid sequence of calf prochymosin. Two clones were identified which together contained a complete copy of prochymosin mRNA. The nucleotide sequence is in substantial agreement with the reported amino acid sequence of prochymosin and shows that this protein has a mol.wt. of 40431 and chymosin a mol.wt. of 35612. The sequence also indicates that prochymosin is synthesised as a precursor molecule, preprochymosin, having a 16 amino acid hydrophobic leader sequence analogous to that reported for other secreted proteins.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cattle Chymosin/genetics Cloning, Molecular DNA DNA Restriction Enzymes Enzyme Precursors/genetics Protein Biosynthesis RNA, Messenger/genetics Stomach/enzymology

Chemicals

Enzyme Precursors RNA, Messenger DNA DNA Restriction Enzymes preprorennin Chymosin

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Harris T J

Lowe P A

Lyons A

Thomas P G

Eaton M A

Millican T A

Patel T P

Bose C C

Carey N H

Doel M T

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.7.2177

Published

1982-04-10

Pages

2177-87

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320601

Subset

Databases

GENBANK

J00003

External Links

PubMed source DOI: 10.1093/nar/10.7.2177 PMC Full Text View journal details

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Molecular cloning and nucleotide sequence of cDNA coding for calf preprochymosin.

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