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PMID: 6283478 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence coding for major outer membrane protein OmpA of Shigella dysenteriae.

Nucleic acids research ·Vol. 10 ·No. 7 ·1982-04-10 ·Pages 2367-78

Braun G, Cole ST

Abstract

The nucleotide sequence of the ompA gene from Shigella dysenteriae has been determined and the amino acid sequence of the pro-OmpA protein predicted. Sequence comparison between the ompA genes of S.dysenteriae and Escherichia coli showed that features such as mRNA secondary structure and codon usage, as well as polypeptide function, have been conserved during evolution. The pro-OmpA protein of S.dysenteriae consists of 351 residues, as opposed to the 346 of the E.coli protein and also shows several amino acid changes. These changes have been used to interpret differences in the biological activity of the two proteins.

MeSH Terms
Amino Acid Sequence Bacterial Outer Membrane Proteins Bacterial Proteins/genetics Base Sequence Biological Evolution Codon/genetics DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Genes Membrane Proteins/genetics Nucleic Acid Conformation Plasmids RNA, Messenger/genetics Shigella dysenteriae/genetics
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Codon DNA, Bacterial Membrane Proteins RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Braun G
Cole S T
References (33)
33 references, click to expand
  1. Complementation analysis of temperature-sensitive host specificity mutations in Escherichia coli.
    J Mol Biol. 1970 May 28;50(1):111-27 PMID: 4916553
  2. Enzyme evolution in the Enterobacteriaceae.
    J Bacteriol. 1972 Jun;110(3):793-802 PMID: 4113124
  3. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  4. Differential inhibitory effects of antibiotics on the biosynthesis of envelope proteins of Escherichia coli.
    J Mol Biol. 1973 Sep 15;79(2):373-89 PMID: 4586413
  5. Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.
    J Bacteriol. 1974 Sep;119(3):726-35 PMID: 4604263
  6. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  7. Covalent lipoprotein from the outer membrane of Escherichia coli.
    Biochim Biophys Acta. 1975 Oct 31;415(3):335-77 PMID: 52377
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  10. Action of a major outer cell envelope membrane protein in conjugation of Escherichia coli K-12.
    J Bacteriol. 1977 Mar;129(3):1651-2 PMID: 321438
  11. Major outer membrane protein d of Escherichia coli K12. Purification and in vitro activity of bacteriophages k3 and f-pilus mediated conjugation.
    FEBS Lett. 1977 Mar 15;75(1):285-90 PMID: 323051
  12. Major proteins of the Escherichia coli outer cell envelope membrane as bacteriophage receptors.
    J Bacteriol. 1977 Sep;131(3):821-9 PMID: 330500
  13. Resistance of Escherichia coli to penicillins: fine-structure mapping and dominance of chromosomal beta-lactamase mutations.
    J Bacteriol. 1977 Oct;132(1):1-7 PMID: 334718
  14. Major outer membrane proteins of Escherichia coli K-12: evidence for protein II being a transmembrane protein.
    FEBS Lett. 1978 Feb 1;86(1):21-4 PMID: 340280
  15. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  16. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  17. Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins.
    J Bacteriol. 1978 Oct;136(1):280-5 PMID: 361695
  18. Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12.
    Mol Gen Genet. 1979 Jun 7;173(2):115-25 PMID: 386030
  19. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  20. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  21. Molecular cloning of the DNA ligase gene from bacteriophage T4. I. Characterisation of the recombinants.
    J Mol Biol. 1979 Aug 15;132(3):471-91 PMID: 160464
  22. DNA sequences from the str operon of Escherichia coli.
    J Biol Chem. 1980 May 25;255(10):4660-6 PMID: 6989816
  23. Nucleotide sequence of the E coli gene coding for dihydrofolate reductase.
    Nucleic Acids Res. 1980 May 24;8(10):2255-74 PMID: 6159575
  24. Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12.
    Nucleic Acids Res. 1980 Jul 11;8(13):3011-27 PMID: 6253901
  25. Regulatory region of the gene for the ompA protein, a major outer membrane protein of Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3845-9 PMID: 7001445
  26. Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4592-6 PMID: 7001461
  27. Cloned structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12: localization on hybrid plasmid pTU100 and expression of a fragment of the gene.
    Mol Gen Genet. 1980;179(1):13-20 PMID: 6256604
  28. Major heat-modifiable outer membrane protein in gram-negative bacteria: comparison with the ompA protein of Escherichia coli.
    J Bacteriol. 1980 Aug;143(2):906-13 PMID: 7009566
  29. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  30. Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction.
    J Mol Biol. 1980 Nov 5;143(3):317-28 PMID: 6260961
  31. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  32. Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens.
    J Bacteriol. 1982 Jan;149(1):145-50 PMID: 7033204
  33. Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors.
    Gene. 1981 Dec;16(1-3):227-35 PMID: 6282694
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.7.2367
Published
1982-04-10
Pages
2367-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320615
Subset
IM
Databases
GENBANK
V01344
External Links
PubMed source DOI: 10.1093/nar/10.7.2367 PMC Full Text View journal details
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