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PMID: 6283552 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human gamma-trace, a basic microprotein: amino acid sequence and presence in the adenohypophysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 3024-7

Grubb A, Löfberg H

Abstract

The amino acid sequence of human gamma-trace, a basic microprotein without known function, was determined by automated Edman degradations of the carboxymethylated polypeptide chain and of fragments obtained by cyanogen bromide treatment and tryptic digestion after blocking of lysine residues. The single polypeptide chain contained 120 residues, and the calculated Mr was 13,260. A proline residue at position 3 was partly hydroxylated. The presence of gamma-trace in a significant proportion of the cells in the anterior lobe of simian and human pituitary glands was demonstrated by immunohistochemical procedures with a rabbit antiserum against human gamma-trace. The tissue localization and amino acid sequence of gamma-trace indicated that this protein is connected with the peptidergic gastroenteropancreatic neuroendocrine system.

MeSH Terms
Adrenocorticotropic Hormone/immunology Amino Acid Sequence Cystatin C Cystatins Globulins/immunology,metabolism Glucagon/immunology Humans Immunoenzyme Techniques Pituitary Gland, Anterior/metabolism
Chemicals
CST3 protein, human Cystatin C Cystatins Globulins Adrenocorticotropic Hormone Glucagon
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grubb A
Löfberg H
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.3024
Published
1982-05-00
Pages
3024-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346341
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.9.3024 PMC Full Text View journal details
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