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PMID: 6284708 Published · ppublish English Journal Article

Cloning and expression of the distal portion of the histidine operon of Escherichia coli K-12.

Journal of bacteriology ·Vol. 151 ·No. 2 ·1982-08-00 ·Pages 692-700

Grisolia V, Carlomagno MS, Bruni CB

Abstract

The operator-distal genes hisBHAFI(E) of the Escherichia coli K-12 histidine operon were mapped on a DNA fragment 4,500 base pairs long. This fragment, originally present in a lambda transducing phage, was cloned in the vector plasmid pBR313. A restriction map was determined, allowing identification of the orientation of the genes in the fragment. The cloned genes were expressed in appropriate hosts, independent of the orientation of the DNA fragment, as shown by transformation tests and by enzyme assays of one of the gene products, hisB, histidinol phosphatase. An internal transcription initiation site was identified by isolation of the cellular RNA, hybridization to specific DNA probes, and mapping by S1 nuclease.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics,metabolism Gene Expression Regulation Histidine/biosynthesis Operon Plasmids Transcription, Genetic
Chemicals
Histidine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grisolia V
Carlomagno M S
Bruni C B
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.2.692-700.1982
Published
1982-08-00
Pages
692-700
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220310
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.2.692-700.1982 PMC Full Text View journal details
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