Home LiteratureArticle Details
PMID: 6285309 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterisation of the Xenopus laevis albumin genes: loss of 74K albumin gene sequences by library amplification.

Nucleic acids research ·Vol. 10 ·No. 9 ·1982-05-11 ·Pages 2791-807

May FE, Weber R, Westley BR

Abstract

The blood of the frog X.laevis contains 2 albumins of 68,000 and 74,000 daltons which are encoded in the liver by two related mRNAs. When an amplified X.laevis DNA library was screened with cloned albumin cDNA only 68,000 dalton albumin gene sequences were isolated. Hybridisation of the albumin cDNA to Southern-blots of Eco R1 digested X.laevis DNA showed that the sequences present in the recombinants did not account for all the fragments which hybridised on the Southern-blots. This indicated that 74K albumin gene sequences exist but that they are not present in the amplified DNA library. A X.laevis genomic library was therefore constructed and screened for albumin genes without amplification. Both 68K and 74K albumin gene sequences were isolated. Recombinants containing 74K albumin gene sequences grew extremely poorly and this probably explains why the 74K albumin sequences were ot isolated from the amplified library. Characterisation of the cloned DNA indicates that there is one sequence coding for the 68K albumin but two different sequences coding for the 75K albumin.

MeSH Terms
Animals Cloning, Molecular DNA/genetics,isolation & purification DNA Restriction Enzymes Female Genes Kinetics Liver/metabolism Molecular Weight Nucleic Acid Denaturation Nucleic Acid Hybridization RNA, Messenger/genetics Serum Albumin/genetics Xenopus
Chemicals
RNA, Messenger Serum Albumin DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
May F E
Weber R
Westley B R
References (17)
17 references, click to expand
  1. Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.
    Eur J Biochem. 1976 Feb 16;62(2):417-23 PMID: 1253796
  2. Albumin phylogeny for clawed frogs (Xenopus).
    Science. 1977 Feb 25;195(4280):785-7 PMID: 65013
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  5. Vitellogenin in Xenopus laevis is encoded in a small family of genes.
    Cell. 1979 Mar;16(3):535-49 PMID: 455441
  6. The rat serum albumin gene: analysis of cloned sequences.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
  7. Rapid DNA isolations for enzymatic and hybridization analysis.
    Methods Enzymol. 1980;65(1):404-11 PMID: 6246361
  8. Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1437-41 PMID: 6445556
  9. Molecular cloning and characterization of the human beta-like globin gene cluster.
    Cell. 1980 Apr;19(4):959-72 PMID: 6155216
  10. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  11. The evolution of alpha-fetoprotein and albumin. II. The structures of the alpha-fetoprotein and albumin genes in the mouse.
    J Biol Chem. 1981 Feb 25;256(4):1960-7 PMID: 6161930
  12. Vitellogenesis and the vitellogenin gene family.
    Science. 1981 Apr 17;212(4492):298-304 PMID: 7209528
  13. Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
    Nucleic Acids Res. 1981 Mar 11;9(5):1069-86 PMID: 6112733
  14. Chromosomal arrangement of the chicken beta-type globin genes.
    Cell. 1981 Jun;24(3):669-77 PMID: 7249078
  15. Xenopus laevis serum albumins are encoded in two closely related genes.
    Nucleic Acids Res. 1981 Aug 11;9(15):3557-74 PMID: 6169009
  16. The endogenous proviral mouse mammary tumor virus genes of the GR mouse are not identical and only one corresponds to the exogenous virus.
    Nucleic Acids Res. 1981 Oct 10;9(19):4981-95 PMID: 6273791
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.9.2791
Published
1982-05-11
Pages
2791-807
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320656
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.9.2791 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2