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PMID: 6285379 Published · ppublish English Journal Article

Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 7 ·1982-04-00 ·Pages 2355-9

Matsumoto K, Uno I, Oshima Y, Ishikawa T

Abstract

Mutants of Saccharomyces cerevisiae that require cAMP for growth have been isolated. Some of the mutants isolated were deficient in adenylate cyclase activity and mapped at a locus, cyr1, located near the centromere of chromosome X. Growth of cells carrying the cyr1 mutation was arrested at the G1 phase of the yeast cell cycle in the absence of cAMP. The cyr1 mutation was suppressed by a secondary mutation designated bcy1. The bcy1 mutation bypassed the need for cAMP for growth. The bcy1 mutants had extremely low levels of cAMP-binding protein and cAMP-dependent protein kinase but produced a high level of cAMP-independent protein kinase. The results indicate that cAMP is an essential factor for yeast cells to proceed through the cell cycle via the activation of protein kinase.

MeSH Terms
Adenylyl Cyclases/genetics Carrier Proteins/genetics Cell Cycle Chromosome Mapping Cyclic AMP Cyclic AMP Receptor Protein Genes Mutation Phenotype Protein Kinases/genetics Saccharomyces cerevisiae/cytology,genetics
Chemicals
Carrier Proteins Cyclic AMP Receptor Protein Cyclic AMP Protein Kinases Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matsumoto K
Uno I
Oshima Y
Ishikawa T
References (27)
27 references, click to expand
  1. Genetical mutants induced by ethyl methanesulfonate in Saccharomyces.
    Can J Genet Cytol. 1965 Sep;7(3):491-9 PMID: 5866861
  2. An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'.
    Nature. 1966 Jul 9;211(5045):206-7 PMID: 5965527
  3. Content of cyclic 3',5'-adenosine monophosphate and adenylyl cyclase in yeast at various growth conditions.
    Biochemistry. 1972 Jul 18;11(15):2788-91 PMID: 4339468
  4. Adenylate cyclase activity in Neurospora crassa. I. General properties.
    J Biol Chem. 1972 Nov 10;247(21):6873-9 PMID: 4343160
  5. Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus.
    J Bacteriol. 1973 Mar;113(3):1240-8 PMID: 4347967
  6. Adenosine 3':5'-monophosphate-dependent protein kinase from yeast.
    J Biol Chem. 1974 Jan 25;249(2):530-5 PMID: 4358557
  7. Saccharomyces cerevisiae cell cycle.
    Bacteriol Rev. 1974 Jun;38(2):164-98 PMID: 4599449
  8. A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity.
    Biochem Biophys Res Commun. 1974 Jun 18;58(4):990-6 PMID: 4365561
  9. Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):442-6 PMID: 164653
  10. Cyclid AMP and catabolite repression in yeasts, In Schizosaccharomyces pombe glucose lowers both intracellular adenosine 3':5'-monophosphate levels and the activity of catabolite-sensitive enzymes.
    Eur J Biochem. 1974 Nov 1;49(1):305-16 PMID: 4156516
  11. Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis.
    J Mol Biol. 1974 Apr 15;84(3):445-61 PMID: 4618856
  12. Respiratory capacity, cyclic adenosine 3',5'-monophosphate, and morphogenesis of Mucor racemosus.
    J Bacteriol. 1975 Oct;124(1):134-9 PMID: 170243
  13. Control of Neurospora crassa morphology by cyclic adenosine 3', 5'-monophosphate and dibutyryl cyclic adenosine 3', 5'-monophosphate.
    J Bacteriol. 1976 Apr;126(1):91-9 PMID: 177398
  14. The effect of glucose and manganese on adenosine-3',5'-monophosphate levels during growth and differentiation of Aspergillus nidulans.
    Arch Microbiol. 1976 May 3;108(1):41-4 PMID: 179489
  15. Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):2995-9 PMID: 184453
  16. Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. II. Solubilization, purification, and some properties of an endogenous adenosine 3':5'-monophosphate-dependent protein kinase.
    J Biol Chem. 1977 Jul 25;252(14):5164-74 PMID: 17614
  17. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
  18. Identification, characterization, and quantitative measurement of cyclic AMP receptor proteins in cytosol of various tissues using a photoaffinity ligand.
    J Biol Chem. 1977 Sep 25;252(18):6494-500 PMID: 197093
  19. Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4.
    J Bacteriol. 1978 May;134(2):446-57 PMID: 207666
  20. Cyclic AMP and cyclic GMP in germinating conidia of Neurospora crassa.
    Arch Microbiol. 1978 Jul;118(1):87-90 PMID: 211974
  21. Exogenous adenosine 3': 5'-monophosphate can release yeast from catabolite repression.
    Biochem Biophys Res Commun. 1978 Aug 14;83(3):1039-47 PMID: 213060
  22. Assay of cyclic nucleotide phosphodiesterase and resolution of multiple molecular forms of the enzyme.
    Adv Cyclic Nucleotide Res. 1979;10:69-92 PMID: 222125
  23. Adenylyl cyclase deficient cr-1 (Crisp) mutant of Neurospora crassa: cyclic AMP-dependent nutritional deficiencies.
    Arch Microbiol. 1979;123(3):251-8 PMID: 230797
  24. Characterization of a cyclic AMP-binding protein from bakers' yeast. Identification as a regulatory subunit of cyclic AMP-dependent protein kinase.
    J Biol Chem. 1980 Mar 10;255(5):2137-45 PMID: 6243658
  25. Yeast mating pheromone alpha factor inhibits adenylate cyclase.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1898-902 PMID: 6246513
  26. Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Feb;1(2):83-93 PMID: 6765598
  27. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.7.2355
Published
1982-04-00
Pages
2355-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346192
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.7.2355 PMC Full Text View journal details
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