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PMID: 6286603 Published · ppublish English Journal Article

A plasmid cloning vehicle for Haemophilus influenzae and Escherichia coli.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1605-7

McCarthy D, Clayton NL, Setlow JK

Abstract

A new plasmid cloning vehicle (pDM2) was used to introduce a library of Haemophilus influenzae chromosomal fragments into H. influenzae. Transformants of the highly recombination-defective rec-1 mutant were more likely to contain exclusively recombinant plasmids after exposure to ligated DNA mixtures than was the wild type. pDM2 could replicate in Escherichia coli K-12.

MeSH Terms
Ampicillin/pharmacology Cloning, Molecular DNA Replication DNA, Recombinant Escherichia coli/genetics Genetic Vectors Haemophilus influenzae/genetics Plasmids Transformation, Bacterial
Chemicals
DNA, Recombinant Ampicillin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McCarthy D
Clayton N L
Setlow J K
References (10)
10 references, click to expand
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  2. Radiation-sensitive and radiation-resistant mutants of Haemophilus influenzae.
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    J Bacteriol. 1968 Nov;96(5):1718-24 PMID: 5303721
  4. A complex of recombination and repair genes in Haemophilus influenzae.
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    J Virol. 1973 Apr;11(4):585-91 PMID: 4540713
  6. Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.
    J Mol Biol. 1975 Jul 25;96(1):171-84 PMID: 169355
  7. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b.
    J Bacteriol. 1976 Apr;126(1):439-46 PMID: 770429
  8. Plasmid transformation in Haemophilus influenzae.
    J Bacteriol. 1981 Feb;145(2):1099-101 PMID: 6970194
  9. Molecular nature of a plasmid specifying beta-lactamase production in Haemophilus ducreyi.
    J Bacteriol. 1981 Dec;148(3):788-95 PMID: 6273382
  10. Transformation of Haemophilus influenzae by plasmid RSF0885 containing a cloned segment of chromosomal deoxyribonucleic acid.
    J Bacteriol. 1981 Dec;148(3):804-11 PMID: 6975774
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.3.1605-1607.1982
Published
1982-09-00
Pages
1605-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220445
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.3.1605-1607.1982 PMC Full Text View journal details
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