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PMID: 6286991 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and characterization of murine leukemia virus-related DNA sequences from C3H/HeN mouse DNA.

Journal of virology ·Vol. 43 ·No. 1 ·1982-07-00 ·Pages 113-26

Roblin R, Young JM, Mural RJ, Bell TE, Ihle JN

Abstract

Ten murine leukemia virus (MuLV)-related DNA sequences were isolated from C3H/HeN mouse genomic DNA by cloning of EcoRI fragments in a Charon 4A vector. Detailed restriction endonuclease maps of four of the clones were developed by using AKR MuLV [32P]cDNA as a probe. C3H clone 14-9 contains approximately 7 kilobase pairs of MuLV-related DNA, one copy of an MuLV long terminal repeat-like sequence, and a region of flanking mouse DNA. C3H clones 34.2 and 36.1 contain approximately 2 kilobase pairs of MuLV-related DNA, one copy of a MuLV LTR-like sequence, and differing lengths of flanking mouse DNA sequences. C3H clone 8.13 was found to contain an insert of 5.7 kilobase pairs of MuLV-related DNA with two long terminal repeat-like regions and sequences which are partially homologous to AKv-1. Comparison fo the restriction endonuclease cleavage maps of these C3H clones with maps recently developed for ecotropic and xenotropic MuLV DNAs indicates that C3H clone 14-9 corresponds to the 5'-terminal portion of a genomic DNA sequence related to xenotropic MuLVs, whereas C3H clones 34.2 and 36.1 correspond to the 3' terminal portions of genomic DNA sequences related to xenotropic MuLVs. Clone 8.13 represents a deleted, xenotropic MuLV-related provirus. C3H clones 14-9, 34.2, 36.1, and 8.13 provide defined DNA sequence probes with which to characterize the organization and expression of endogenous MuLV-related DNA sequences in the mouse genome.

MeSH Terms
Animals Base Sequence Chromosomes/analysis Cloning, Molecular DNA Restriction Enzymes DNA, Viral Leukemia Virus, Murine/genetics Mice Mice, Inbred C3H/genetics,microbiology Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roblin R
Young J M
Mural R J
Bell T E
Ihle J N
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.1.113-126.1982
Published
1982-07-00
Pages
113-26
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256102
Subset
IM
Grants
NCI NIH HHS · N01-CO-75380 · United States
External Links
PubMed source DOI: 10.1128/JVI.43.1.113-126.1982 PMC Full Text View journal details
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