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PMID: 6288330 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromosomal arrangement of heat shock locus 2-48B in Drosophila hydei.

Chromosoma ·Vol. 85 ·No. 2 ·1982-00-00 ·Pages 237-49

Peters FP, Grond CJ, Sondermeijer PJ, Lubsen NH

Abstract

cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arrangements, as shown by restriction mapping, in our wild type D. hydei population. These genomic arrangements are allelic. Both alleles contain multiple copies of the N09-15 sequence but differ in their lengths and in the distribution of Msp I and Taq I sites.

MeSH Terms
Alleles Animals Base Sequence Chromosomes/physiology DNA/metabolism DNA Restriction Enzymes Drosophila/genetics Embryo, Nonmammalian/physiology Heat-Shock Proteins Hot Temperature Nucleic Acid Hybridization Plasmids Proteins/genetics
Chemicals
Heat-Shock Proteins Proteins DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peters F P
Grond C J
Sondermeijer P J
Lubsen N H
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00294968
Published
1982-00-00
Pages
237-49
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00294968 View journal details
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