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PMID: 6288664 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and identification of the product of the dnaE gene of Escherichia coli.

Journal of bacteriology ·Vol. 152 ·No. 1 ·1982-10-00 ·Pages 351-6

Welch MM, McHenry CS

Abstract

We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli chromosome was determined. A unique HindIII restriction endonuclease site within the cloned segment of DNA was identified as a site required for expression of the dnaE gene. By using the maxicell plasmid-directed protein synthesizing system, we demonstrated that dnaE codes for the alpha subunit of DNA polymerase III.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Chromosomes, Bacterial Cloning, Molecular DNA Polymerase III/genetics DNA Restriction Enzymes DNA-Directed DNA Polymerase/genetics Deoxyribonuclease HindIII Escherichia coli/genetics Genes, Bacterial Plasmids
Chemicals
Bacterial Proteins DNA Polymerase III DNA-Directed DNA Polymerase DNA Restriction Enzymes Deoxyribonuclease HindIII
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Welch M M
McHenry C S
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22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.1.351-356.1982
Published
1982-10-00
Pages
351-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221416
Subset
IM
Grants
PHS HHS · AU833 · United States
NIGMS NIH HHS · GM 25457 · United States
External Links
PubMed source DOI: 10.1128/jb.152.1.351-356.1982 PMC Full Text View journal details
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