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PMID: 6289267 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Ribosomal RNA genes in Mycoplasma.

Nucleic acids research ·Vol. 10 ·No. 14 ·1982-07-24 ·Pages 4215-22

Amikam D, Razin S, Glaser G

Abstract

Using Southern blotting analysis with labelled mycoplasmal ribosomal RNA as probe, two fragments (1 Kb and 5 Kb) were detected in an EcoR I digest of Mycoplasma capricolum DNA. This analysis revealed that the 5 Kb fragment carries both 16S rRNA sequences and the entire 23S rRNA gene of this mycoplasma. The 1 Kb fragment contains 16S rRNA sequences only. The 5 Kb EcoR I fragment has been cloned and used to characterize the structure of rRNA cistrons in various Mycoplasma strains. These experiments clearly demonstrate a substantial homology of Mycoplasma capricolum rRNA sequences with the E. coli rRNA cistron on one hand, and with Mycoplasma mycoides subsp. capri and Acholeplasma laidlawii on the other hand. This analysis also reveals two rRNA cistrons in Mycoplasma mycoides subsp. capri and Acholeplasma laidlawii whereas one rRNA cistron is present in Mycoplasma capricolum.

MeSH Terms
Acholeplasma laidlawii/genetics Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Genes Mycoplasma/genetics Nucleic Acid Hybridization RNA, Ribosomal/genetics Species Specificity
Chemicals
DNA, Bacterial RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Amikam D
Razin S
Glaser G
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.14.4215
Published
1982-07-24
Pages
4215-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320794
Subset
IM
Grants
NIGMS NIH HHS · GM 25286 · United States
External Links
PubMed source DOI: 10.1093/nar/10.14.4215 PMC Full Text View journal details
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