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PMID: 6290458 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.

Journal of bacteriology ·Vol. 152 ·No. 2 ·1982-11-00 ·Pages 924-7

Nano FE, Kaplan S

Abstract

The transposon Tn951 (lac) was introduced into the photosynthetic bacterium Rhodopseudomonas sphaeroides 2.4.1, which is normally Lac-, via the P-group plasmid RP1. beta-Galactosidase was produced constitutively in both chemotrophically and phototrophically grown cells, and the levels were found to be the same but low. Mutants were isolated, however, that were able to grow on lactose minimal medium and which expressed different levels of beta-galactosidase when grown chemotrophically or phototrophically. The beta-galactosidase levels found in all R. sphaeroides strains were much less than those found in Escherichia coli.

MeSH Terms
DNA Transposable Elements Genotype Mutation Operon Phenotype Plasmids Rhodobacter sphaeroides/genetics beta-Galactosidase/genetics
Chemicals
DNA Transposable Elements beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nano F E
Kaplan S
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.2.924-927.1982
Published
1982-11-00
Pages
924-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221553
Subset
IM
Grants
NIGMS NIH HHS · GM 7283 · United States
External Links
PubMed source DOI: 10.1128/jb.152.2.924-927.1982 PMC Full Text View journal details
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