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PMID: 6290999 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a highly repetitive family of DNA sequences in the mouse.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 5003-13

Fanning TG

Abstract

A large proportion (0.5-1%) of total mouse DNA is cleaved by Bam HI into fragments whose size is about 500 base pairs. A cloned member of this repetitive family of DNA sequences (BAM5 family) was sequenced by the dideoxy chain termination procedure and shown to contain 507 base pairs. The sequence exhibited no unusual or remarkable features. Repetitive sequences complementary to the cloned BAM5 fragment were found in rat DNA, but not in feline or human DNA. Restriction mapping suggested that many BAM5 sequences were components of much larger repetitive DNAs which were scattered throughout the mouse genome. The BAM5 sequences within the larger repetitive DNAs did not appear to be arranged tandemly or as members of scrambled tandem repeats. RNA homologous to the cloned BAM5 sequence was detected in cultured mouse cells, but not in cultured rat cells.

MeSH Terms
Animals Cats Cell Line Cloning, Molecular DNA/genetics DNA Restriction Enzymes Deoxyribonuclease BamHI Humans Mice Mice, Inbred Strains Rats Repetitive Sequences, Nucleic Acid Species Specificity
Chemicals
DNA DNA Restriction Enzymes Deoxyribonuclease BamHI
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fanning T G
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.16.5003
Published
1982-08-25
Pages
5003-13
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320847
Subset
IM
Grants
NCI NIH HHS · 5 RO1 CA 21454 · United States
Databases
GENBANK
J00633
External Links
PubMed source DOI: 10.1093/nar/10.16.5003 PMC Full Text View journal details
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