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PMID: 6291035 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5240-4

Kurilla MG, Piwnica-Worms H, Keene JD

Abstract

The leader RNA transcript from the 3' end of the genome of vesicular stomatitis virus (VSV) has been detected in both the nucleus and cytoplasm of infected baby hamster kidney (BHK) cells. In the cytoplasm, leader RNA accumulated gradually throughout the infection to about 200 molecules per cell at 6 hr after infection. In the nucleus, however, there was a sharp and rapid increase in the concentration of leader RNA to approximately equal to 300 molecules per cell at about 2 hr after infection that decreased rapidly by 3 hr. This report presents evidence for nuclear localization of transcription products of a (-)-strand RNA virus other than influenza and supports the hypothesis that the leader RNA plays a role in the shutoff of host cell transcription.

MeSH Terms
Animals Base Sequence Cell Line Cell Nucleus/analysis Cell Transformation, Viral Cricetinae Cytoplasm/analysis Kidney Nucleic Acid Hybridization RNA, Viral/biosynthesis,genetics Transcription, Genetic Vesicular stomatitis Indiana virus/genetics
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kurilla M G
Piwnica-Worms H
Keene J D
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.17.5240
Published
1982-09-00
Pages
5240-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346871
Subset
IM
Grants
NCI NIH HHS · 5T32 CA09111 · United States
NCI NIH HHS · P01 CA30246 · United States
NIAID NIH HHS · R01 AI16099 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.17.5240 PMC Full Text View journal details
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