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PMID: 6291060 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mapping of polypeptides encoded by the Epstein-Barr virus genome in productive infection.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 18 ·1982-09-00 ·Pages 5698-702

Hummel M, Kieff E

Abstract

Over 30 viral-specified polypeptides are translated in vitro from RNA of cells productively infected with Epstein-Barr virus (EBV). The polypeptides map to sites in EBV DNA by hybrid selection. Almost all of the polypeptides are reactive with EBV immune human serum. Several of the polypeptides are part of the early antigen complex. Two others are likely to be major structural components of the virus. Genes encoding persistent early and late polypeptides are intermixed through most of the EBV genome.

MeSH Terms
DNA Restriction Enzymes DNA, Viral/genetics Electrophoresis, Polyacrylamide Gel Genes, Viral Herpesvirus 4, Human/genetics Humans Molecular Weight Peptides/genetics Protein Biosynthesis RNA, Viral/genetics Viral Proteins/genetics
Chemicals
DNA, Viral Peptides RNA, Viral Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hummel M
Kieff E
References (35)
35 references, click to expand
  1. Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis.
    J Virol. 1981 Jun;38(3):996-1004 PMID: 7017165
  2. Discordant expression of 2 Epstein-Barr virus-associated antigens, EBNA and RANA, in man-rodent somatic cell hybrids.
    J Immunol. 1981 Aug;127(2):585-90 PMID: 6265553
  3. Epstein-Barr virus membrane antigens: characterization, distribution, and strain differences.
    J Virol. 1981 Jul;39(1):172-84 PMID: 6268807
  4. Herpes simplex virus type 1 HindIII fragment L encodes spliced and complementary mRNA species.
    J Virol. 1981 Aug;39(2):559-72 PMID: 6268843
  5. Epstein-Barr virus polypeptides: identification of early proteins and their synthesis and glycosylation.
    J Virol. 1981 Aug;39(2):651-5 PMID: 6268851
  6. Expression of Epstein-Barr viral early antigen in monolayer tissue cultures after transfection with viral DNA and DNA fragments.
    J Virol. 1981 Dec;40(3):861-9 PMID: 6275121
  7. The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets.
    Cell. 1981 Nov;27(1 Pt 2):121-31 PMID: 7326748
  8. Epstein-Barr virus RNA. VIII. Viral RNA in permissively infected B95-8 cells.
    J Virol. 1982 Jul;43(1):262-72 PMID: 6180174
  9. Location of the structural genes for glycoproteins gD and gE and for other polypeptides in the S component of herpes simplex virus type 1 DNA.
    J Virol. 1982 Jul;43(1):41-9 PMID: 6287015
  10. Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.
    Mol Cell Biol. 1981 Sep;1(9):785-96 PMID: 9279391
  11. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus.
    Science. 1970 Jul 10;169(3941):188-90 PMID: 4316788
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. Appearance of Epstein-Barr virus-associated antigens in infected Raji cells.
    Virology. 1971 Jul;45(1):10-21 PMID: 4329584
  14. Synthesis of Epstein-Barr virus after activation of the viral genome in a "virus-negative" human lymphoblastoid cell (Raji) made resistant to 5-bromodeoxyuridine (thymidine kinase-virus antigen-immunofluorescence-herpesvirus fingerprints).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):78-82 PMID: 4550511
  15. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
    Int J Cancer. 1973 May;11(3):499-520 PMID: 4133943
  16. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344-8 PMID: 1093178
  17. Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus.
    J Virol. 1976 Mar;17(3):935-49 PMID: 176452
  18. Proteins of Epstein-Barr Virus. II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus.
    J Virol. 1976 Apr;18(1):289-97 PMID: 176465
  19. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  20. Quantitative analysis of specific labelled RNA'S using DNA covalently linked to diazobenzyloxymethyl-paper.
    Nucleic Acids Res. 1979 Jan;6(1):195-203 PMID: 218170
  21. Tumor initiators and promoters in the induction of Epstein-Barr virus.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):782-5 PMID: 218219
  22. Analysis of early and late Epstein-Barr virus associated polypeptides by immunoprecipitation.
    Virology. 1979 Sep;97(2):378-87 PMID: 224588
  23. Polypeptides of the Epstein-Barr virus membrane antigen complex.
    J Virol. 1979 Nov;32(2):458-67 PMID: 228070
  24. Immunochemical characterization of Epstein-Barr virus-associated early and late antigens in n-butyrate-treated P3HR-1 cells.
    J Virol. 1979 Dec;32(3):710-6 PMID: 229261
  25. Epstein-Barr virus-induced proteins. III. Analysis of polypeptides from P3HR-1-EBV-superinfected NC37 cells by immunoprecipitation.
    Virology. 1980 Apr 15;102(1):231-3 PMID: 6245505
  26. Radioimmune precipitation study comparing the Epstein-Barr virus membrane antigens expressed on P3HR-1 virus-superinfected Raji cells to those expressed on cells in a B-95 virus-transformed producer culture activated with tumor-promoting agent (TPA).
    Virology. 1980 Apr 30;102(2):360-9 PMID: 6245514
  27. Epstein-Barr virus-induced proteins. II. Analysis of surface polypeptides from EBV-producing and -superinfected cells by immunoprecipitation.
    Virology. 1980 Apr 30;102(2):401-11 PMID: 6154378
  28. Detection of virus-specific antigens in EB-(P3HR1) virus-superinfected Raji cells by immunoprecipitation.
    Virology. 1980 Jun;103(2):340-9 PMID: 6247820
  29. Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 PMID: 6248879
  30. DNA of Epstein-Barr virus VIII: B95-8, the previous prototype, is an unusual deletion derivative.
    Cell. 1980 Nov;22(1 Pt 1):257-67 PMID: 6253079
  31. Epstein-Barr virus-induced polypeptides: a comparative study with superinfected Raji, IUdR-Treated, and N-butyrate-treated P3HR-1 cells.
    Virology. 1981 Feb;109(1):72-81 PMID: 6258314
  32. Epstein-Barr virus polypeptides: effect of inhibition of viral DNA replication on their synthesis.
    J Virol. 1981 Jan;37(1):61-71 PMID: 6261001
  33. Regulation of herpesvirus macromolecular synthesis: transcription-initiation sites and domains of alpha genes.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7122-6 PMID: 6261240
  34. Epstein-Barr virus RNA VII: size and direction of transcription of virus-specified cytoplasmic RNAs in a transformed cell line.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1930-4 PMID: 6112750
  35. Epstein-Barr virus RNA. VI. Viral RNA in restringently and abortively infected Raji cells.
    J Virol. 1981 May;38(2):649-60 PMID: 6165836
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.18.5698
Published
1982-09-00
Pages
5698-702
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346972
Subset
IM
Grants
NCI NIH HHS · CA 17281 · United States
NCI NIH HHS · CA 19264 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.18.5698 PMC Full Text View journal details
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