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PMID: 6292474 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antibodies against a synthetic peptide of the poliovirus replicase protein: reaction with native, virus-encoded proteins and inhibition of virus-specific polymerase activities in vitro.

Journal of virology ·Vol. 43 ·No. 3 ·1982-09-00 ·Pages 969-78

Baron MH, Baltimore D

Abstract

A carboxy-terminal peptide of the poliovirus replicase protein (p63) was chemically synthesized, coupled to bovine serum albumin carrier, and injected into rabbits. The resulting antisera reacted with six virus-specific proteins from HeLa cells infected with poliovirus: NCVP 0b, NCVP 1b, NCVP 2, a protein of about 60,000 daltons, p63, and NCVP 6b. The identity of the 60,000-dalton protein is not known, but the other results were consistent with previous experimental approaches which demonstrated that p63 and the other four polypeptides have common coding sequences. An amino-terminal peptide of p63 failed to elicit an immune response in rabbits. Antibodies raised against the p63 carboxy-terminal peptide inhibited poliovirus replicase and polyuridylic acid polymerase activities in vitro, providing strong support for earlier suggestions that these activities are a property of a single virus-specific polypeptide.

MeSH Terms
Animals Antibodies, Viral/immunology Antigen-Antibody Reactions Humans Nucleotidyltransferases/immunology Peptides/chemical synthesis,immunology Poliovirus/enzymology,immunology RNA Nucleotidyltransferases/immunology RNA-Dependent RNA Polymerase/immunology Rabbits Viral Proteins/immunology
Chemicals
Antibodies, Viral Peptides Viral Proteins Nucleotidyltransferases RNA Nucleotidyltransferases poly U polymerase RNA-Dependent RNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baron M H
Baltimore D
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41 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.3.969-978.1982
Published
1982-09-00
Pages
969-78
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256207
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.3.969-978.1982 PMC Full Text View journal details
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