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PMID: 6292514 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effect of aphidicolin on avian sarcoma virus replication.

Journal of virology ·Vol. 44 ·No. 2 ·1982-11-00 ·Pages 493-8

Hsu TW, Taylor JM

Abstract

We studied the effect of aphidicolin, an inhibitor of eucaryotic DNA polymerase alpha, on viral DNA replication and integration during the first 24 h after infection of quail embryo fibroblasts with avian sarcoma virus. In drug-treated cells, the synthesis of unintegrated linear viral DNA species was not impaired; however, the subsequent accumulation of circular viral DNA species and integrated proviral DNA was reversibly inhibited. After removal of the drug, circular viral DNA species were derived from preexisting linear viral DNA species, instead of being derived by de novo synthesis.

MeSH Terms
Animals Aphidicolin Avian Sarcoma Viruses/drug effects,physiology Cells, Cultured DNA/biosynthesis DNA Replication/drug effects DNA, Circular/biosynthesis DNA, Viral/biosynthesis Diterpenes/pharmacology Quail Recombination, Genetic/drug effects Virus Replication/drug effects
Chemicals
DNA, Circular DNA, Viral Diterpenes Aphidicolin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hsu T W
Taylor J M
References (22)
22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.44.2.493-498.1982
Published
1982-11-00
Pages
493-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256292
Subset
IM
Grants
ODCDC CDC HHS · CC-22651 · United States
NCRR NIH HHS · RR-05539 · United States
External Links
PubMed source DOI: 10.1128/JVI.44.2.493-498.1982 PMC Full Text View journal details
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