Rauscher murine leukemia virus: molecular cloning of infectious integrated proviral DNA.
The integrated proviral genome of Rauscher murine leukemia virus was molecularly cloned in a bacteriophage Charon 4A vector after the proviral sequences were enriched by sequential RPC-5 column chromatography and sucrose gradient centrifugation. A recombinant DNA clone, lambda-RV-1, possessing a 12-kilobase-pair EcoRI insert, was shown to contain the entire 8.8-kilobase-pair leukemia virus genome flanked by rat cellular sequences at the 5' and 3' ends. This DNA fragment was biologically active, inducing the release of virion-associated reverse transcriptase activity with as little as 10 ng of DNA insert. The virus induced XC plaque formation at high titers on NIH/3T3 and BALB/3T3 cells and demonstrated identity with the parental virus in radioimmunoassays for the highly type-specific gag gene-coded p12 protein. The molecularly cloned Rauscher murine leukemia virus should be useful in studying the molecular mechanisms involved in the transformation of specific lymphoid target cells by chronic mouse leukemia viruses.
28 references, click to expand
-
Selective extraction of polyoma DNA from infected mouse cell cultures.J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
-
Plaque assay techniques for murine leukemia viruses.Virology. 1970 Dec;42(4):1136-9 PMID: 4099080
-
A new technique for the assay of infectivity of human adenovirus 5 DNA.Virology. 1973 Apr;52(2):456-67 PMID: 4705382
-
Characterization of temperature-sensitive mutants of murine leukemia virus.Virology. 1973 Jul;54(1):53-9 PMID: 4123462
-
Analysis of type specific antigenic determinants of two structural polypeptides of mouse RNA C-Type viruses.Virology. 1974 Mar;58(1):1-8 PMID: 4132402
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
Radiommunoassays for the 70,000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera.J Virol. 1976 Jun;18(3):933-41 PMID: 58081
-
Screening lambdagt recombinant clones by hybridization to single plaques in situ.Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
-
Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.Proc Natl Acad Sci U S A. 1977 Oct;74(10):4406-10 PMID: 270684
-
In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.Gene. 1977 May;1(3-4):255-80 PMID: 338419
-
The gene order of avian RNA tumor viruses derived from biochemical analyses of deletion mutants and viral recombinants.Annu Rev Microbiol. 1978;32:561-92 PMID: 213012
-
The integrated genome of murine leukemia virus.Cell. 1978 Nov;15(3):1003-10 PMID: 728982
-
Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA.Cell. 1978 Dec;15(4):1383-95 PMID: 215324
-
Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates.J Virol. 1978 Dec;28(3):810-8 PMID: 215781
-
Restriction enzyme analysis of mouse cellular type C viral DNA: emergence of new viral sequences in spontaneous AKR/J lymphomas.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1677-81 PMID: 221902
-
In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus.Cell. 1979 Apr;16(4):863-74 PMID: 88264
-
Multiple integration sites for Moloney murine leukemia virus in productively infected mouse fibroblasts.J Virol. 1979 Jun;30(3):657-67 PMID: 480464
-
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
-
The transforming gene of Moloney murine sarcoma virus.Nature. 1979 Nov 22;282(5737):378-83 PMID: 503218
-
Different lymphoid cell targets by transformation by replication-competent Moloney and Rauscher mouse leukemia viruses.Cell. 1980 Mar;19(3):663-9 PMID: 6767548
-
Nucleotide sequence of Moloney leukemia virus: 3' end reveals details of replications, analogy to bacterial transposons, and an unexpected gene.Proc Natl Acad Sci U S A. 1980 Jun;77(6):3302-6 PMID: 6251454
-
Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41 PMID: 6254003
-
Nucleotide sequence analysis of the long terminal repeat (LTR) of avian retroviruses: structural similarities with transposable elements.Cell. 1980 Nov;22(2 Pt 2):379-86 PMID: 6256079
-
Avian leukosis virus-induced tumors have common proviral integration sites and synthesize discrete new RNAs: oncogenesis by promoter insertion.Cell. 1981 Feb;23(2):323-34 PMID: 6258798
-
Isolation of recombinant DNA clones carrying complete integrated proviruses of Moloney murine leukemia virus.J Virol. 1981 Jan;37(1):181-90 PMID: 6260972
-
Activation of a cellular onc gene by promoter insertion in ALV-induced lymphoid leukosis.Nature. 1981 Apr 9;290(5806):475-80 PMID: 6261142
-
Complete nucleotide sequence and organization of the Moloney murine sarcoma virus genome.Science. 1981 Oct 23;214(4519):445-50 PMID: 6170110
-
Multiple arrangements of viral DNA and an activated host oncogene in bursal lymphomas.Nature. 1982 Jan 21;295(5846):209-14 PMID: 6276760
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
