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PMID: 6292844 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA polymerase alpha from the nuclear matrix of cells infected with simian virus 40.

Nucleic acids research ·Vol. 10 ·No. 18 ·1982-09-25 ·Pages 5517-32

Jones C, Su RT

Abstract

The nuclear matrix prepared from normal, simian virus 40 (SV40)-infected, and SV40-transformed cells contained DNA polymerase activities. Approximately 12% of the total DNA polymerase activities in isolated nuclei remained with the nuclear matrix. alpha-polymerase was the major matrix DNA polymerase activity as judged by sensitivity to various inhibitors: aphidicolin, dideoxy-TTP, and N-ethylmaleimide. Approximately 2-4 fold higher DNA polymerase activity was detected in matrices obtained from lytically infected and virus-transformed cells than that found in normal cells. In lytically infected cells, 30-50% of the matrix-bound DNA polymerase activity solubilized by sonication co-sedimented with majority of the matrix T-antigen, and was co-precipitated with anti-T sera. The results suggest that alpha-polymerase and viral T-antigen may form a functional complex in the matrix.

MeSH Terms
Animals Cell Line Cell Nucleus/enzymology Cell Transformation, Viral Chlorocebus aethiops Cricetinae DNA Polymerase II/genetics,metabolism DNA-Directed DNA Polymerase/genetics Embryo, Mammalian Fibroblasts/enzymology Kidney Rats Simian virus 40/enzymology,genetics
Chemicals
DNA Polymerase II DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones C
Su R T
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39 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.18.5517
Published
1982-09-25
Pages
5517-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320895
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.18.5517 PMC Full Text View journal details
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