pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.
A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment.
10 references, click to expand
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DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
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Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.Gene. 1977;2(2):75-93 PMID: 344136
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Amino acid sequence of beta-galactosidase. XI. Peptide ordering procedures and the complete sequence.J Biol Chem. 1978 Aug 10;253(15):5521-5 PMID: 97298
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A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
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The rapid purification of T4 DNA ligase from a lambda T4 lig lysogen.J Biol Chem. 1980 Feb 10;255(3):813-5 PMID: 6985906
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Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.J Mol Biol. 1979 Aug 15;132(3):493-505 PMID: 392109
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Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
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Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.Mol Gen Genet. 1980;178(2):475-7 PMID: 6248731
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A system for shotgun DNA sequencing.Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
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pUR222, a vector for cloning and rapid chemical sequencing of DNA.Nucleic Acids Res. 1981 Aug 25;9(16):4087-98 PMID: 6272193
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