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PMID: 6292870 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of intron size on splicing efficiency in retroviral transcripts.

Nucleic acids research ·Vol. 10 ·No. 19 ·1982-10-11 ·Pages 6177-90

Cullen BR, Kopchick JJ, Stacey DW

Abstract

To study the effect of intron size on splicing efficiency we have varied the size of the avian leukosis virus (ALV) env mRNA intron in a cloned ALV genome. This was accomplished by deletion of ALV sequences or insertion of phage lambda DNA. The effect of these modifications on splicing was analyzed by microinjection of the modified clones into RSV(-) chicken cells. Viral env mRNA when transcribed and properly spliced within these cells complemented the RSV(-) env deficiency leading to the production of focus forming units. Using this assay it was shown that deletion of up to 3.7 kb of the 4.68 kb env intron did not inhibit correct splicing nor did insertion of up to 8 kb of phage lambda DNA prevent splicing. Our results indicate that intron size can be varied over a wide range without preventing splicing.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Cell Transformation, Viral Chick Embryo Cloning, Molecular DNA Restriction Enzymes Fibroblasts Mutation Parainfluenza Virus 1, Human/genetics Plasmids RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cullen B R
Kopchick J J
Stacey D W
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.19.6177
Published
1982-10-11
Pages
6177-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320959
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.19.6177 PMC Full Text View journal details
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