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PMID: 6293469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of 'catalytic' iron and anti-oxidant activity in extracellular fluids.

The Biochemical journal ·Vol. 206 ·No. 3 ·1982-09-15 ·Pages 605-9

Gutteridge JM, Rowley DA, Halliwell B

Abstract

Synovial fluid from rheumatoid patients and normal cerebrospinal fluid contains micromolar concentrations of non-protein-bound iron salts that can promote lipid peroxidation and also the superoxide-dependent formation of hydroxyl radicals from hydrogen peroxide. These iron catalysts of oxygen radical reactions cannot be detected by conventional assays unless interfering high-molecular-weight substances, probably proteins, are removed by ultrafiltration or inactivated by exposure to low pH values. The bleomycin assay for ;catalytic' iron [Gutteridge, Rowley & Halliwell (1981) Biochem. J.199, 263-265] does not suffer from these artifacts.

MeSH Terms

Animals Brain/drug effects Cattle Free Radicals Humans Iron/analysis,metabolism,pharmacology Lipid Metabolism Lipids/analysis Liposomes/metabolism Oxidation-Reduction Oxygen/metabolism Superoxides/analysis,metabolism Synovial Fluid/analysis

Chemicals

Free Radicals Lipids Liposomes Superoxides Iron Oxygen

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gutteridge J M

Rowley D A

Halliwell B

References (27)

27 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2060605

Published

1982-09-15

Pages

605-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1158629

Subset

External Links

PubMed source DOI: 10.1042/bj2060605 PMC Full Text View journal details

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Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of 'catalytic' iron and anti-oxidant activity in extracellular fluids.

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