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PMID: 6294471 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of the metJBLF gene cluster of Escherichia coli in lambda met transducing phage.

Molecular & general genetics : MGG ·Vol. 187 ·No. 3 ·1982-00-00 ·Pages 401-4

Greene RC, Krueger JH, Johnson JR

Abstract

The position of the metJBLF gene cluster in the transducing phage lambda met102 was determined by ligation of its leftmost EcoRI fragment (102-1) to the lambda BCDEF (nin5) EcoRI fragment of lambda gtl (lambda BC) and characterization of the resultant recombinant phage. The new transducing phage carries about 6kb of bacterial DNA which contains the entire met gene cluster including the promoter of its rightmost member metF. Reasonable estimates of the coding capacity required for the four genes indicate that most of the bacterial DNA of the recombinant phage is occupied by the met gene cluster.

MeSH Terms
Bacteriophage lambda/enzymology,genetics Cystathionine beta-Synthase/genetics Cystathionine gamma-Lyase/genetics DNA Restriction Enzymes Deoxyribonuclease EcoRI Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genes, Viral Methionine Adenosyltransferase/genetics Methylenetetrahydrofolate Dehydrogenase (NADP)/genetics Nucleic Acid Hybridization Transduction, Genetic
Chemicals
Methylenetetrahydrofolate Dehydrogenase (NADP) Methionine Adenosyltransferase DNA Restriction Enzymes Deoxyribonuclease EcoRI Cystathionine beta-Synthase Cystathionine gamma-Lyase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Greene R C
Krueger J H
Johnson J R
References (17)
17 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332618
Published
1982-00-00
Pages
401-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM10317 · United States
External Links
PubMed source DOI: 10.1007/BF00332618
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